OBJECTIVE： To screen the optimal technology of dog testes and penis processed with talcum powder， and to provide reference for the formulation of quality control standard. METHODS： Taking the comprehensive score of crushing rate and the content of alcohol soluble extract as evaluation indexes， L9（34） orthogonal test was used to optimize processing temperature， processing time and the amount of talcum powder； validation test was conducted. According to related method in Chinese Pharmacopoeia （2015 edition）， the contents of moisture， ash， nitrogen and alcohol soluble extract were determined， and automatic amino acid analyzer was used to determine the content of amino acids in samples. RESULTS： The optimal processing technology were that 100 kg dog testes and penis should add into 40 kg talcum powder； the processing temperature was 350-380 ℃； processing lasted for 4 min. Results of verification test showed that the average crushing rate and the content of alcohol soluble extract were 80.2％ （RSD＝0.95％， n＝3） and 15.7％ （RSD＝2.30％， n＝3）. In processed dog testes and penis， the contents of moisture， ash， nitrogen and alcohol soluble extract were 5.7％， 18.7％， 8.3％， 15.7％， respectively. Besides， there still were 16 kinds of amino acids， and their total content was 42.44％. CONCLUSIONS： The optimized talcum powder processing technology of dog testes and penis is stable and practical. The content of moisture， ash， nitrogen， alcohol soluble extract and amino acid can be used as important quality standard control indexes for processed dog testes and penis.