OBJECTIVE： To establish a method for simultaneous determination of gallic acid and ellagic acid in Xihe majun ointment. METHODS： HPLC method was performed on the column of X-Bridge C18 with mobile phase of methanol-acetonitrile-0.2％ Phosphoric acid solution （gradient elution） at a flow rate of 1.0 ml/min， detection wavelength was 254 nm，detection temperature was 30 ℃， and injection volume was 10 μl. RESULTS： The linear range was 0.132-0.792 μg for gallic acid （r＝0.999 9） and 0.108 0-0.648 2 μg for ellagic acid （r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 3％； recoveries were 95.15％-99.69％（RSD＝1.64％，n＝9） and 97.24％-100.43％（RSD＝1.24％，n＝9）. CONCLUSIONS： The method is simple， accurate and sensitive， and can be used for the content determination of gallic acid and ellagic acid in Xihe majun ointment.