OBJECTIVE：To establish a method for simultaneous determination of calycosin-7-glucoside， specnuezhenide， berberine hydrochloride， palmatine hydrochloride and rosmarinic acid in Xialiqi capsule. METHODS： UPLC was performed on the column of Shield RP18 with mobile phase of nitrile -0.2％ formic acid （gradient elution） at a flow rate of 0.2 ml/min， the column temperature was 35 ℃， the detection wavelength was 240 nm， and the injection volume was 1 μl. RESULTS： The linear range was 0.619-39.6 μg/ml for calycosin-7-glucoside （r＝0.999 9）， 1.181-115.6 μg/ml for specnuezhenide （r＝0.999 9），0.89-57.2 μg/ml for berberine hydrochloride（r＝0.999 9），1.16-74 μg/ml for palmatine hydrochloride（r＝0.999 9） and 2.47-157.8 μg/ml for rosmarinic acid（r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 3％， recoveries were 97.98％-102.44％（RSD＝1.49％，n＝9）， 97.37％-100.96％（RSD＝1.13％，n＝9）， 98.53％-102.30％ （RSD＝1.40％，n＝9）， 97.06％-102.21％（RSD＝1.46％，n＝9） and 100.10％-102.25％（RSD＝0.68％，n＝9）， respectively. CONCLUSIONS： The method is simple， rapid and reproducible， and can be used for the quality control of Xialiqi capsule.