OBJECTIVE： To provide suggestions for improving the quality of the instructions of domestic generic Levofloxacin injections. METHODS： A set of instructions of original Levofloxacin injection and 20 sets of instructions of domestic generic Levofloxacin injections （with three kinds of acid radicals） were compared and analyzed in warning， indications， dosage and administration， medication for children， drug interactions， toxicology， pharmacokinetics， etc. RESULTS & CONCLUSIONS： The rate of recording indications， dosage and administration and pharmacokinetics among the 21 sets of instructions is high， up to 100％. Among the instructions of domestic generic Levofloxacin hydrochloride injection， Levofloxacin lactate injection and Levofloxacin mesylate injection， the rates of mentioning a tendon injury in the warning section are 30.00％， 0 and 33.33％ respectively； those of recording antibacterial spectrum in the indications section are 10.00％， 0 and 33.33％ respectively； those of recording the specific results of drug interactions are 10.00％， 0 and 66.67％ respectively； and those of recording toxicological information are 70.00％， 85.71％ and 33.33％ respectively， all of which are lower than the recording rates of 100％ for the instructions of original one. In the instructions of domestic generic Levofloxacin injection， there is a lack of clinical trial data in the sections of dosage and administration， medication for children and toxicology and of pharmacokinetic parameters for patients with impaired liver/kidney function and special population in the pharmacokinetics section. It is suggested that the manufacturers of domestic generic Levofloxacin injections should improve the specific contents of the instructions； the instructions regulatory authorities should improve the administration of instructions examination and approval； and doctors and pharmacists should strictly follow the instructions.