OBJECTIVE: To establish the quality standard of Flemingia philippinensis. METHODS: The properties and microscopic characteristics were observed; TLC was adopted for qualitative identification of genistin and genistein; the moisture, total ash and extract were detected; HPLC was adopted for contents determination of genistin and genistein:the column was Thermo BETASIL C18 with mobile phase of Acetonitrile -0.5% glacial acetic acid (gradient elution) at a flow rate of 1.0 ml/min, the detection wavelength was 261 nm, column temperature was 25 ℃, and the injection volume was 10 μl. RESULTS: The properties and microscopic characteristics of F. philippinensis and F. macrophylla showed strong specificity. TLC spots of genistin and genistein we- re clear and well separated, with no interference in negative control. The moisture was 3.69%-8.37%,total ash was 1.72%-6.74%, and extract was 5.89%-19.65%. The linear range was 0.012 9-2.588 μg for genistin (r=0.999 9) and 0.004 6-0.923 2 μg for genistein (r=0.999 9); RSDs of precision, stability and reproducibility tests were lower than 3.0%; recoveries were 99.63%-101.87% (RSD=0.82%,n=6) and 97.19%-100.34% (RSD=1.23%,n=6). CONCLUSIONS: The method is simple, accurate and specific, and can be used for the quality control of F. philippinensis.