OBJECTIVE： To establish a method for the determination of related substances in Levo-cetirizine hydrochloride chewable tablet. METHODS： RP-HPLC was performed on the column of Venusil XBP-CN with mobile phase of acetonitrile-0.05 mol/L Sodium dihydrogen phosphate solution（pH value adjusted to 3.0 by phosphoric acid）（60 ∶ 40，V/V） at a flow rate of 1.0 ml/min， the detection wavelength was 230 nm， column temperature was 25 ℃， and volume injection was 20 μl. RESULTS： Levo-cetirizine hydrochloride chewable tablet showed good separation with related substances； the linear range of levo-cetirizine hydrochloride was 1.2-2.8 μg/ml （r＝0.999 9）； the quantification limit and detection limit was 1.4 ng/ml and 0.3 ng/ml， respectively； RSDs of precision， stability and reproducibility tests were lower than 1％； recovery was 96.3％-105.0％ （RSD＝1.7％，n＝9）； the contents of related substances were no more than 0.17％. CONCLUSIONS： The method is simple， sensitive， reproducible， accurate and reliable， and can be used for the determination of related substances in Levo-cetirizine hydrochloride chewable tablet.