OBJECTIVE： To establish a method for the determination of ethanol， acetonitrile， dichloromethane， ethyl acetate， pyridine in vidarabine monophosphate. METHODS： Headspace GC was performed on the column of Agilent DB-624， programmed temperature， inlet temperature was 200 ℃， the detector was flame ionization detector， detecting temperature was 250 ℃， nitrogen was carrier gas， flow rate was 3 ml/min， split ratio was 1 ∶ 1， the top bottles equilibrium temperature was 100 ℃， and equilibrium time was 45 min， injection volume was 1 ml. external standard was used for quantitative analysis. RESULTS： The peaks of five residual solvents could be completely separated from the other peaks respectively，The linear rang was 24.7-296.3 μg/ml for ethanol（r＝0.999 6）、1.9-23.2 μg/ml for acetonitrile（r＝0.999 0）， 2.8-33.6 μg/ml for dichloromethane（r＝0.998 0）， 24.7-295.9 μg/ml for ethyl acetate（r＝0.999 5）， 1.0-11.9 μg/ml for pyridine（r＝0.998 6）； RSDs of precision and reproducibility tests were lower than 4.35％；recoveries were 102.4％（RSD＝2.0％，n＝9）、102.1％（RSD＝3.4％，n＝9）、105.5％（RSD＝4.8％，n＝9）、100.3％（RSD＝4.8％，n＝9）、98.3％（RSD＝4.0％，n＝9）. The minimum quantifation limit was 0.304 4-0.988 0 μg/ml and the minimum detection limit was 0.101 5-0.329 3 μg/ml. CONCLUSIONS： The method is simple， accurate and reproducible， and can be used for the determination of residual solvents in vidarabine monophosphate.