OBJECTIVE： To prepare Levofloxacin and triamcinolone acetonide double-loaded ophthalmic gel. METHODS： Using levofloxacin hydrochloride and triamcinolone acetonide as main components， carbopol-940P as base material， HPMC K4M as tackifier， Levofloxacin and triamcinolone acetonide double-loaded ophthalmic gel was prepared. Using dissolution time as index， the contents of carbopol-940P and HPMC K4M were determined by single factor test， and dissolution time， viscosity and the contents of 2 main components were also determined. RESULTS： The concentrations of carbopol-940P and HPMC K4M were 0.4％ and 1.2％， separately. The dissolution time was more than 24 h and viscosity was 1 142.67 Pa·s. The content of levofloxacin hydrochloride was 97.3％ of labelled amount （RSD＝0.84％， n＝3）， and that of triamcinolone acetonide was 92.97％ of labelled amount （RSD＝1.32％， n＝3）. CONCLUSIONS： Levofloxacin and triamcinolone acetonide double-loaded ophthalmic gel has been prepared successfully.