OBJECTIVE： To establish a method for the content determination of amygdalin in Lianhua qingwen capsule. METHODS： HPLC was performed on the column of Phenomenex Kinetex XB-C18 with mobile phase of acetonitrile-0.2％ Phosphoric acid solution （6 ∶ 94，V/V）at a flow rate of 1.0 ml/min， the detection wavelength was 207 nm， column temperature was 30 ℃， and the injection volume was 10 μl. RESULTS： The linear range of amygdalin was 43.16-215.80 μg/ml （r＝0.999 7）； the limit of detection was 0.431 6 μg/ml，the limit of quantitation was 1.294 8 μg/ml； RSDs of precision， stability and reproducibility tests no more than 0.69％； recovery was 95.16％-100.49％ （RSD＝1.67％，n＝9）. CONCLUSIONS： The method is simple and rapid with high accuracy and well reproducibility， and can be used for the content determination of amygdalin in Lianhua qingwen capsule.