OBJECTIVE： To establish a method for the content determination of verbascoside in Naosaitong honeyed pill. METHODS： HPLC was performed on the column of Zorbax ODX with mobile phase of acetonitrile -0.1％ phosphoric acid（16 ∶ 84， V/V） at a flow rate of 1.0 ml/min， the detection wavelength was 334 nm， column temperature was 30 ℃， and the injection volume was 10 μl. RESULTS： The linear range of verbascoside was 0.200-1.000 μg（r＝0.999 8）； RSDs of precision， stability and reproducibility tests were lower than 1％； recovery was 95.16％-99.78％（RSD＝1.80，n＝6）. CONCLUSIONS： The method is simple and accurate， and suitable for the content determination of verbascoside in Naosaitong honeyed pill.