OBJECTIVE： To investigate the improvement effects of Zhuanggushenjin capsules on osteoporosis induced by retinoic acid in mice. METHODS： 84 mice were randomly divided into normal control group， model control group， positive control group [Gushukang granules， 5 g（crude drug）/kg] and Zhuanggushenjin capsules high-dose， medium-dose and low-dose groups [7.80， 3.90， 1.95 g（crude drug）/kg]. Except for normal control group， those groups were given retinoic acid （90 mg/kg） to induce osteoporosis model. 7 days after modeling， all drug groups were given relevant medicine intragastrically， normal control group and model control group given normal saline intragastrically ，once a day， for consecutive 14 days. ALP activity and the contents of calcium and phosphorus in serum and thigh-bone were determined. The body weight， the dry weight， length and diameter of thigh-bone were determined， and organ coefficients （spleen， testicle， ovarium and uterus） were calculated. RESULTS： Compared with normal control group， the activity of ALP in serum of mice increased in model control group， while the contents of calcium and phosphorus， body weight， length and diameter of thigh-bone decreased significantly； spleen coefficients increased， while testicle and uterus coefficients decreased （P＜0.05 or P＜0.01）. Compared with model control group， the contents of calcium and phosphorus increased in Zhuanggushenjin capsules groups， while spleen coefficient decreased； the activity of ALP in serum of mice decreased in high-dose and medium-dose groups， while body weight， the length of thigh-bone， testicle and uterus coefficients increased； diameter of thigh-bone increased in high-dose group （P＜0.05 or P＜0.01）. CONCLUSIONS： Zhuanggushenjin capsules can improve retinoic acid-induced osteoporosis in mice to certain extent.