OBJECTIVE： To establish the quality standard of Huoxuesan. METHODS： Microscopic identification method was adopted for the qualitative identification of Rhizoma pinelliae， Eupolyphaga seu， Phellodendron amurense and Fructus kochiae in the preparation. HPLC was adopted for the contents determination of phellodendrine，polydatin， jatrorrhizine， berberine and emodin： the column was ZORBAX  Eclipse  Plus C18 with methanol-0.1％ phosphoric acid（gradient elution）at a flow rate of 0.4 ml/min， the detection wavelength was 240 nm， column temperature was 30 ℃， and the injection volume was 5 μl. RESULTS： Microscopic identification map of R. pinelliae， E. seu， P. amurense and F. kochiae was clear.  The linear range was  0.159-5.073 μg（r＝0.997 4）for phellodendrine、0.149-4.761 μg（r＝0.999 9）for polydatin、0.239-7.649 μg（r＝0.995 5）for jatrorrhizine、0.165-5.268 μg（r＝0.997 2）for berberine、0.012-0.382 μg（r＝0.999 9）for emodin； RSDs of precision， stability and reproducibility tests were lower than 3.0％； recoveries were 98.9％-104.1％（RSD＝1.9％，n＝6）， 96.1％-101.7％（RSD＝2.5％，n＝6）， 99.6％-105.1％ （RSD＝2.6％，n＝6）， 90.3％-98.2％ （RSD＝2.9％，n＝6） and 92.9％-96.4％ （RSD＝2.0％，n＝6） respectively. CONCLUSIONS： The established standard can be used for the quality control of Huoxuesan.