OBJECTIVE： To establish a method for the contents determination of ginsenoside Rg1， ginsenoside Re， ginsenoside Rb1 and geniposide in Panax ginseng processed by Gardenia jasminoides. METHODS： HPLC was performed on the column of Agilent TC18 with mobile phase of acetonitrile-0.1％ phosphoric acid， （gradient elution， ginsenoside Rg1， ginsenoside Re， ginsenoside Rb1） and acetonitrile-water （13 ∶ 87，V/V， geniposide） at a flow rate of 1.0 ml/min， the detection wavelength was 203 nm for ginsenoside Rg1， ginsenoside Re， ginsenoside Rb1 and 238 nm for geniposide， column temperature was 40 ℃， and injection volume was 20 μl. RESULTS： The linear range was 0.418-3.762 μg for ginsenoside Rg1（r＝0.999 8）， 0.270-2.430 μg for Re（r＝0.999 8），0.398-3.582 μg for ginsenoside Rb1（r＝0.999 7） and 0.606-3.030 μg for geniposide（r＝0.999 7）； RSDs of precision， stability and reproducibility tests were less than 2.0％；recoveries were 97.86％-101.47％（RSD＝1.29％，n＝6）， 96.21％-100.11％ （RSD＝1.42％，n＝6）， 96.24％-100.48％（RSD＝1.57％，n＝6） and 97.76％-102.44％ （RSD＝1.71％，n＝6）. CONCLUSIONS： The method is simple with good precision， stability and reproducibility， and can be used for the contents determination of ginsenoside Rg1， ginsenoside Re， ginsenoside Rb1 and geniposide in P. ginseng processed by Gardenia jasminoides.