OBJECTIVE： To study the effects and mechanism of rapamycin on invasion and metastasis of cervical cancer HeLa cell. METHODS： HeLa cells were divided into control group and rapamycin low-dose， medium-dose and high-dose groups （10， 30， 100 nmol/L）. After treated for 48 h， cell viability was measured by MTT assay， and inhibitory rate was calculated； migration and invasion of cell was tested by Transwell assay. The expression of matrix metalloproteinase 2 （MMP-2）， MMP-9， Vimentin and E-cadherin， and phosphorylation of protein kinase B （Akt）， mammalian target of rapamycin （mTOR） were detected by Western blot. RESULTS： Compared with control group， the inhibition rate of cell viability was increased in rapamycin groups （P＜0.01）； the number of invasion and metastasis cells decreased （P＜0.01）； the expression of MMP-2， MMP-9 and Vimentin were decreased （P＜0.01 or P＜0.05）； the expression of E-cadherin was enhanced （P＜0.01 or P＜0.05）； the phosphorylation of Akt and mTOR were reduced （P＜0.01）. CONCLUSIONS： Rapamycin could inhibit invasion and metastasis of HeLa cell via Akt/mTOR signal pathway.