OBJECTIVE：To establish a method for simultaneous determination for ginsenoside Rb1， ginsenosides Rc， ginsenosides Rb2 and ginsenosides Rd in Shengmai powder. METHODS：HPLC was performed on the column of Agilent ZORBAX Extend-C18 with mobile phase of acetonitrile -0.1％ formic acid（gradient elution） at a flow rate of 1.0 ml/min， wavelength was 203 nm， column temperature was 30 ℃ and the volume injection was 10 ml. RESULTS：The linear was 0.78-38.75 μg for ginsenoside Rb1（r＝0.999 6），0.51-25.50 μg for ginsenosides Rc （r＝0.999 6），0.43-21.50 μg for ginsenosides Rb2（r＝0.999 7） and 0.20-10.00 μg for ginsenosides Rd（r＝0.999 7）； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 96.6％-101.2％（RSD＝1.6％，n＝6），97.0％-102.1％（RSD＝1.7％，n＝6），99.1％-102.8％（RSD＝1.3％，n＝6） and 96.3％-101.1％（RSD＝1.6％，n＝6）. CONCLUSIONS：The method is simple， rapid with good stability， reproducibility and high precision， and can be used for quality control of Shengmai powder.