OBJECTIVE：To optimize and improve the quality standards of Huangpu weining granule. METHODS：TLC was used for qualitative identification of Coptis chinensis， Pericarpium citri， Leguminosae， Herba taraxaci， Bletilla striata， Bupleurum chinense and Radix glycyrrhizae. HPLC was used for the content determination of berberine hydrochloride. Column was Dikma Diamonsil C18 with mobile phase of acetonitrile-0.05 mol/L Potassium dihydrogen phosphate solution （50 ∶ 50，V/V）（per 100 ml was added sodium lauryl sulfate 0.4 g， and then adjusted the pH to 4.0 with phosphoric acid） at a flow rate of 1.0 ml/min， the detection wavelength was 345 nm， the column temperature was 30 ℃， and the injection volume was 10 μl. RESULTS： The TLC of C. chinensis， P.citri， Leguminosae， H. taraxaci， B. striata，B. chinense and R. glycyrrhizae showed clear spots and good separation. The linear range of berberine hydrochloride was 2.03-81.2 μg/ml（r＝0.999 9）；RSDs of precision， stability and reproducibility tests were lower than 1％； recovery was 99.67％-103.04％（RSS＝1.14，n＝9）. CONCLUSIONS：The optimized and improved method is helpful for the quality control of Huangpu weining granule.