OBJECTIVE： To establish a method for the determination of clopidogrel in human plasma， and to study the bioequvivalence of 2 kinds of Clopidogrel hydrogen sulfate tablets. METHODS： 60 healthy male volunteers were randomly divided into group A， B， C and D. By four cycles repeated cross-over design， they were given fasting （group A and B） and postprandial （group C and D） test or reference preparation 75 mg. The concentration of clopidogrel in human plasma was determined by LC-MS/MS. Using diazepam as internal standard， Ultimate XB-C18 column was adopted with mobile phase consisted of acetonitrile-0.05％ formic acid （84 ∶ 16，V/V）. Positive ion detection was conducted in MRM mode， and monitoring transition ion-pair was m/z 322.00→212.00 for clopidogrel and m/z 285.00→154.00 for internal standard. Pharmacokinetic parameters were calculated by using DAS 3.2.8 software， and two-way and unilateral t test and non-parameters test were used to investigate the bioequivalence of two preparations. RESULTS： The linear rang of clopidogrel was 20-5 000 pg/ml. The pharmacokinetic parameters of test preparation or reference preparation after fasting oral administration were as follows： tmax were（0.850±0.356） and （0.764±0.336）h； cmax were （1 514.550± 1 120.469） and （1 506.900±1 054.008）pg/ml； t1/2 were （4.036±3.615） and （4.056±4.193）h； AUC0-t were （2 721.814±2 062.832） and （2 826.051±2 526.683） pg·h/ml. Those of test preparation or reference preparation after postprandial oral administration were as follows： tmax were （1.513±0.709） and （1.558±0.743）h； cmax were （5 219.509±2 711.907） and （5 520.337±3 336.664） pg/ml； t1/2 were （5.800±4.729） and （5.513±4.206）h； AUC0-t were （10 890.81±4 101.357）and（11 350.653±4 174.848） pg·h/ml. Under two kinds of condition， 90％CI of AUC0-t， AUC0-∞ and cmax of test preparations were 80％-125％ of corresponding parameters of reference preparations， there was no statistical significance in tmax between two preparations（P＞0.05）. CONCLUSIONS： The method is rapid， sensitive with no endogenous interference. Two tablets are bioequivalent.