OBJECTIVE： To optimize the formulation of Asiaticoside cationic liposomes， and to investigate the characteristics of drug release in vitro. METHODS： The thin film dispersion method was used to prepare liposome； using encapsulation efficiency and drug-loading amount as index， the formulation of Asiaticoside liposomes was optimized by central composite design-response surface method with the ratio of drug to lipid （X1）， the ratio of cholesterol to lipid （X2） and the concentration of D-mannose （X3） as factors. Using sodium lauryl sulfate as medium， in vitro release characteristics of cationic liposomes prepared with 1％ octadecylamine was investigated by bag filter method， and compared with those of Asiaticoside solution and common liposome. RESULTS： The optimal formulation was X1 0.07， X2 0.17 and X3 0.03 g/ml. The encapsulation efficiency was （75.529±1.071）％， and drug-loading amount was （2.539±0.029）％ （n＝3）； the deviation from the predicted values were －0.217％ and 0.205％； 1％ octadecylamine was add into formulation to obtain cationic liposomes， and the Zeta potential had changed from －5.6 mV to 20.8 mV. in vitro accumulative release rates of Asiaticoside solution， common liposomes and cationic liposomes were 89.13％（12 h）， 87.58％（72 h） and 94.46％（72 h）， and the latter was in line with Weibull model. CONCLUSIONS： Asiaticoside cationic liposomes have high encapsulation efficiency， and can releases for 72 h.