OBJECTIVE：To establish a method for simultaneous determination of oridonin， ponicidin and rosemary acid in Donglingcao tablet. METHODS： With the reference peak of oridonin， HPLC with changingwavelength method was conducted to calculate the relative correction factors of ponicidin and rosemary acid， which were used to calculate the contents of ponicidin and rosemary acid. The column was Phenomenex C18 with mobile phase of acetonitrile - 0.2％ phosphoric acid （gradient elution） at a flow rate of 1.0 ml/min， detection wavelength was 239 nm（0-20 min，ponicidin），329 nm（20-25 min，rosemary acid），239 nm（25-49 min，oridonin），the column temperature was 40 ℃，and the injection volume was 10 μl. RESULTS： The linear range was 41.6-623.4 ng（r＝0.999 9） for oridonin，13.8-207.2 ng（r＝0.999 9） for ponicidin， 36.8-552.0 ng（r＝0.999 9） for rosemary acid. RSDs of precision， stability and reproducibility tests were lower than 3％， recovery was 99.6％-102.5％（RSD＝1.1％，n＝6）、98.8％-101.4％（RSD＝1.0％，n＝6）、99.0％-102.5％（RSD＝1.5％，n＝6）. CONCLUSIONS： The method is simple， stable and reproducible， and can be used for the simultaneous determination of oridonin， ponicidin and rosemary acid in Donglingcao tablet.