OBJECTIVE： To establish a method for the content determination of paeoniflorin in Bazhen pill （concentrated pill）. METHODS： HPLC was performed on the column of wondasil C18 with mobile phase of acetonitrile -1％ phosphoric acid （15 ∶ 85， V/V） at a flow rate of 1.0 ml/min， the detection wavelength was 230 nm， and column temperature was 30 ℃. RESULTS： The linear range of paeoniflorin was 0219-1.32 μg（r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 1％； recovery was 95.82％-101.82％ （RSD＝2.13％，n＝6）. CONCLUSIONS： The method is simple， stable and reproducible， and can be used for the content determination of Bazhen pill （concentrated pill）.