OBJECTIVE： To monitor the distribution and tendency of multi-drug resistance bacterial in ICU of 16 hospitals from Mianyang city of Sichuan province， and to provide basis for controlling outbreaks and prevalence of multi-drug resistance bacterial and empirical use of antibacterial in this area. METHODS： According to isolated non-repetitive infection multi-drug resistance bacterial from ICU of involved hospital during Jan. 2013 to Dec. 2014， the data were screened and analyzed. RESULTS： A total of 3 578 strains were isolated from ICU of 16 hospitals in Mianyang city from 2013 to 2014. Top 5 multi-drug resistant bacterial were Bauman acinetobacter， ESBLs-producing Klebsiella pneumoniae， Pseudomonas aeruginosa， ESBLs-producing Escherichia coli and Methicillin-resistant Staphylococcus aureus （MRSA）. During 2013-2014， detection rate of B. acinetobacter increased from 2.62％ to 3.31％ （P＜0.05）， and resistance rate of it to ampicillin sodium and sulbactam sodium and compound sulfapirazinmetossina decreased obviously； detection rate of ESBLs-producing K. pneumonia increased from 1.10％ to 1.69％ （P＜0.05）， and resistance rate of it to various antibiotics changed slightly； detection rate of P. aeruginosa decreased from 2.81％ to 2.57％， and resistance rate of it to cephalosperin and quinolone decreased to certain extent； detection rate of ESBLs-producing E. coli increased from 7.93％ to 8.86％ （P＜0.05）， and resistance rate of it to various antibiotics changed slightly； detection rate of MRSA decreased from 0.91％ to 0.81％， and resistance rate of it to penicillin increased to certain extent but to other antibiotics decreased at different levels. CONCLUSIONS： From 2013 to 2014， there was higher detection rate of ESBLs-producing E. coli in ICU of 16 Hospitals from Mianyang city. B. acinetobacter， ESBLs-producing K. pneumoniae and ESBLs-producing E. coli infection showed the overall upward trend. Resistance rate of 5 kinds of multi-drug resistant bacterial to part commonly used antibiotics shows upward trend. Multi-drug resistant bacterial has become an important pathogen of nosocomial infection， and the hospitals at all levels should be strengthened ICU monitoring， use antibiotic drugs rationally， and contain the production of multiple drug resistant strains.