OBJECTIVE：To establish a method for the contents determination of paeoniflorin， hesperidin， baicalin， palmatine， berberine， glycyrrhizic acid， magnolol in Xianglian huazhi pill. METHODS： HPLC was conducted on the column of Agilent Porlshell 120 SB-C18 with mobile phase of 90％ acetonitrile （A）-0.1％ phosphoric acid 5％ aqueous acetonitrile （B） （gradient elution） at a flow rate 1.0 ml/min， the column temperature was 30 ℃， the injection volume was 3 μl， and the detection wavelength was 230 nm for paeoniflorin （0-10.0 min）， 283 nm for hesperidin （10.1-13.0 min）， 277 nm for baicalin （13.1-14.4 min）， 265 nm for palmatine and berberine （14.5-20 min）， 250 nm for glycyrrhizic acid （20.1-25.0 min） and 290 nm for magnolol （25.1-60.0 min）. RESULTS： The linear range was 0.013 88-0.694 5 μg for paeoniflorin， 0.039 93-1.996 8 μg for hesperidin， 0.070 08-0.389 3 μg for baicalin， 0.006 48-0.324 0 μg for palmatine（counted by palmatine hydrochloride），0.010 52-0.526 2 μg for berberine（counted by berberine hydrochloride），0.008 816-0.440 7 μg for glycyrrhizic acid（counted by ammonium glycyrrhizinate） and 0.007 224-0.361 2 μg for magnolol （r≥0.999 0）； RSDs of precision， stability and reproducibility tests were lower than 2.0％； average recoveries were 97.47％-102.88％（RSD＝1.91％，n＝6），102.06％-102.81％（RSD＝0.25％，n＝6），97.91％-100.80％ （RSD＝1.25％，n＝6），97.53％-101.60％（RSD＝1.66％，n＝6），97.54％-100.68％（RSD＝1.15％，n＝6），96.23％-99.00％（RSD＝1.11％，n＝6） and 97.91％-101.44％（RSD＝1.36％，n＝6）， respectively. CONCLUSIONS： The method is simple， rapid and accurate， and can be used for the quality control of Xianglian huazhi pill.