OBJECTIVE： To observe the protective effect of Shuganning injection against liver injury induced by cisplatin in mice. METHODS： 100 mice were randomized into normal control group （normal saline）， model group， Shuganning injection high-dose， medium-dose and low-dose groups [41.5， 20.8， 10.4 g （crude drug）/kg] with 20 mice in each group. Except normal control group， other groups were given cisplatin 3 mg/kg， ip， on Monday and Thursday to induce cisplatin toxicosis model； at the same time， they were given relevant medicines from Monday to Friday by intraperitoneal injection， once a day for consecutive 10 weeks. The activities of ALT and AST， the contents of albumin， total protein and globin in serum， total protein/globin （A/G） were determined， and hepatic index was calculated and hepatic histomorphology was observed. RESULTS： Compared with normal control group， the activity of AST， the contents of ALB and globin in serum and A/G all increased in model group （P＜0.01）， unclear hepatic cords range， hepatocellular albuminoid degeneration and endochylema puffing were common and obvious acidophilic change was found in hepatic tissue. Compared with model group， the serum content of ALB decreased in Shuganning injection low-dose and high-dose groups， while globin content and A/G increased； and the activity of AST in serum decreased in Shuganning high-dose group （P＜0.05 or P＜0.01）， and pathological state of hepatic tissue was improved. CONCLUSIONS： Shuganning injection possesses a protective effect on cisplatin-induced liver injury in mice.