OBJECTIVE：To establish a method for the content determination of psoralen in Psoralea-pericarpium juglates liniment. METHODS： HPLC was performed on the column of Shim-pack VP-ODS C18 with mobile phase of methanol-water （60 ∶ 40，V/V）at a flow rate of 0.8 ml/min， detection wavelength was 246 nm，column temperature was 25 ℃， and injection volume was 20 μl. RESULTS： The linear range of psoralen was 2.49-39.90 μg/ml； RSDs of precision， stability and reproducibility tests were lower than 2.00％； recovery was 97.23％-100.58％ （RSD＝1.17％，n＝6）. CONCLUSIONS：The method is fast， simple， accurate and reliable， and can be used for the content determination of psoralen in Psoralea-pericarpium juglates liniment.