OBJECTIVE：To provide reference for benign sustainable development of traditional Chinese and ethnic medicine enterprises in Guizhou. METHODS：Taking 129 traditional Chinese and ethnic medicine enterprises of 9 city and prefecture in Guizhou as survey object，questionnaires survey was conducted in 55 enterprises by stratified sampling according to location. The data statistical analysis was carried out in respects of related policies familiarity，product positioning，independent innovation from 2009 to 2013. Relevant development countermeasures were put forward. RESULTS & CONCLUSIONS：45.1％ of enterprises expressed to know and be familiar with relevant policies，51.0％ expressed to alert but only understand，and 3.9％ said largely unknown. At the past five years，the annual output value and sales revenue of enterprises had risen year by year. Almost 80％ production and technical personnel of enterprises had the junior title or no title；marketing department staff mainly had college and secondary school degree，accounting for 47.5％ and 25.3％ respectively；research personnel mainly had bachelor and college degree，accounting for 57.0％ and 33.7％ respectively. At the past five years，independent innovation input took up 2.4％ of sales revenue averagely. There are problems，such as small and medium enterprises occupying dominate position；enterprises lacking of sufficient understanding on related policies；strategies being adjusted slowly；independent innovation input intensity needing to increase. Countermeasures and suggestions are put forward，such as strengthening the relevant policy advocacy，guiding corporate mergers and acquisitions，closely paying attention to industry development，changing innovation ideas，and attaching importance to research of exclusive production of essential medicine.