OBJECTIVE：To establish a method for the content determination of carbohydrates in Osseous and cucumis extract injection. METHODS：Ion chromatography was conducted on the column of Dionex CarboPac PA20 with mobile phase of water-1 mol/L Sodium acetate solution-250 mmol/L Sodium hydroxide solution（gradient elution）at flow rate of 0.45 ml/min，column temperature was 35 ℃，and injection volume was 10 μl，detector was electrochemical analysis detector. RESULTS：The linear ranges of galactose，glucose，mannose，sucrose and fructose were 0.5-10.0 μg/ml（r＞0.998 0）；RSDs of precision，stability and reproducibility tests were lower than 2％；recovery was 94.8％-99.1％（RSD＝0.52％-0.89％，n＝9）. CONCLUSIONS：The method is simple， fast and accurate，and can be used for the determination of carbohydrates in Osseous and cucumis extract injection.