OBJECTIVE： To systematically review the efficacy and safety of Calf spleen extract injection combined with chemotherapy in the treatment of cancer， and provide evidence-based reference for clinical treatment. METHODS： Retrieved from Cochrane Library， PubMed， Medline， EMBase， CJFD， Wanfang and VIP Database， randomized controlled trials （RCT） of the efficacy and safety about Calf spleen extract injection combined with chemotherapy in the treatment of cancer were collected. Meta-analysis was performed by using Rev Man 5.0 software after data extract and quality evaluation by Cochrane 5.0. RESULTS： Totally 23 RCT were enrolled， including 1 682 patients. Results of Meta-analysis showed Calf spleen extract injection combined with chemotherapy in the treatment of cancer can significantly improve the effective rate [OR＝2.17，95％CI（1.68，2.81），P＜0.001] and improvement rate of life quality [OR＝4.26，95％ CI（2.47，7.32），P＜0.001] and also reduce the degree rate of WBC and PLT，the incidence rate of nausea and emesis， the differences were statistically significant. CONCLUSIONS： Calf spleen extraction injection combined with chemotherapy has good efficacy and safety in the treatment of cancer.