OBJECTIVE：To observe therapeutic efficacy and safety of carboprost tromethamine in the treatment of postpartum hemorrhage with two routes of administration. METHODS：285 patients with postpartum hemorrhage were randomly divided into control group（143 cases）and trial group（142 cases）. Control group was given Carboprost tromethamine injection 250 μg on deltoid of arm；trial group was given same dose of Carboprost tromethamine injection via cervix uteri. Both groups received medicine after third stage of labor. The therapuetic efficacy，the amount of endometrorrhagia and colporrhagia within 2 h，the incidence of ADR after labor were observed in 2 groups. RESULTS：After treatment，the amount of postpartum hemorrhage was smaller than 400 ml in 2 groups and didn’t exceed the standard，without statistical significance（P＞0.05）. The amount of endometrorrhagia in trial group was significantly higher than in control group，with statistical significance（P＜0.05）；the amount of colporrhagia in trial group was significantly lower than in control group，with statistical significance（P＜0.05）. There was no statistical significance in the incidence of ADR between trial group（4.90％）and control group（6.31％）（P＞0.05）. CONCLUSIONS：The intramuscular injection is selected for the prevention of postpartum hemorrhage caused by uterine inertia；the cervical injection is selected for the prevention of postpartum hemorrhage caused for the other patients.