OBJECTIVE：To study the flavonoids in She medicine Eupatorium chinense. METHODS：Silica gel，ODS and Sephadex LH-20 column chromatography were conducted to isolate and purify the flavonoids in She medicine E. chinense，and compound structures were analyzed and identified based on the physicochemical properties and spectral data. RESULTS：From the ethyl acetate extract of E. chinense，10 flavonoids were isolated as tricin （1），quercetin（2），kaempferol（3），luteolin（4），luteolin- 7-O-β-D-glucoside（5），4-methoxyctricin（6），quercetin-3-O-β-D-glucoside（7），kaempferol-3-O-β-D-glucoside（8），kaempferol- 3-O-rutinoside（9）and rutin（10）. CONCLUSIONS：Compound 1-10 are isolated from E. chinense for the first time. The study provides certain basis for the quality evaluation of E. chinense.