OBJECTIVE：To provide reference for better improving the risk perception of Omeprazole sodium for injection in nurses in a third-grade class-A hospital，strengthening risk management of ward drugs and ensuring safety of patients. METHODS： Nurses in the clinical departments in a third-grade class-A hospital where usage frequency of Omeprazole sodium for injection was relatively more were adopted as study subjects and a self-designed questionnaire was used to investigate on related knowledge on medication risk in nurses in emergency department，gastroenterology department，ICU and CCU in 2012 and 2014. RESULTS：51 and 81 questionnaires were distributed in 2012 and 2014，and 51 and 81 were effectively received with effective recovery of 100％ and 100％，respectively. In both surveys，100％ surveyed nurses could correctly choose one solvent at least，1.96％ and 3.70％ nurses could correctly choose 2 solvents；correct answering rates of intravenous administration time were 94.12％ and 96.30％，respectively； correct answering rates of stable duration after preparation were both lower than 9％；correct answering rates of adverse reaction of this drug were 3.92％ and 1.23％ ，respectively；correct answering rates of interaction of this drug were 1.96％ and 6.17％，respectively；correct answering rates of stable formulation of this drug were 17.65％ and 22.22％，respectively；the proportion of nurses observing ADRs were 25.49％ and 13.58％，respectively；the proportion of nurses finding inappropriate compatibility were 15.69％ and 22.22％，respectively，Ornidazole and sodium chloride injection was the most used incompatible drug；45％ or more nurses hoping to strengthen the continuing education of nursing personnel. CONCLUSIONS：Nurses in this third-grade class-A hospital lack certain knowledge on the medication risk of Omeprazole sodium for injection，the drug risk management is not sufficient，and awareness prevention of drug risk is relative weak. It is suggested that the hospital should strengthen drug risk management system，nurses should strengthen the learning about drug risk and play the role of clinical pharmacists in the drug risk management to reduce the related drug risk and incidence of adverse reactions.