OBJECTIVE：To establish a method for the contents determination of cholic acid，hyodeoxycholic acid and deoxycholate acid in Naoxueshuan tablet. METHODS：HPLC-ELSD was performed on the column of SHIMADZU VP-ODS C18 with mobile phase of acetonitrile-0.2％ formic acid（gradient elution）column temperature was 30 ℃，at flow rate of 1.0 ml/min，and the volume injection was 10 μl. RESULTS：The linear range was 0.039 84-0.398 4 mg for cholic acid，0.032 064-0.320 64 mg for hyodeoxycholic acid and 0.0339 52-0.339 52 μg for deoxycholate acid（r≥0.999 0），and the logarithm value of volume injection and peak area showed good linear relationship；RSDs of precision，stability and reproducibility tests were lower than 2％；recoveries were 101.33％-104.05％（RSD＝0.95％，n＝6），95.56％-101.38％（RSD＝2.54％，n＝6）and 96.07％-97.12％（RSD＝0.44％，n＝6），respectively. CONCLUSIONS：The method is simple and accurate，and suitable for the contents determination of cholic acid，hyodeoxycholic acid and deoxycholate acid in Naoxueshuan tablet.