OBJECTIVE：To optimize the content determination conditions of total alkaloids from Zhuang medicine Munronia delavayi，and to determine the content of total alkaloids in M. delavayi from different producing areas. METHODS：With the content of total alkaloids as index，using solvent amount，ultrasonic time，ultrasonic extraction times and pH value of buffer as factors， the content determination conditions of total alkaloids from Zhuang medicine M. delavayi were optimized by orthogonal test. Optimized content determination conditions were adopted to determine the content of total alkaloids in M. delavayi from 18 producing areas in different harvest time. RESULTS：The optimum content determination conditions were as follows as the amount of solvent（ CHCl3）20 ml，ultrasonic processing for 3 times，lasting for 15 min each time，pH value of buffer 4.5. The contents of total alkaloids in M. delavayi from 18 producing areas were between 0.6-11.98 mg/g，showing great difference. M. delavayi from Longlin county and Tianlin county harvested in Oct. had the lowest content of total alkaloids. CONCLUSIONS：Optimized content determination condition can be used for the content determination of total alkaloids in Zhuang medicine M. delavayi and quality control of it. The content determination of total alkaloids in M. delavayi is related to producing area and harvest time.