OBJECTIVE：To optimize the inclusion technology of Eugenol-β-cyclodextrin（β-CD）inclusion complex，and to identify and characterize it. METHODS：With the molar ratio of eugenol to β-CD，inclusion temperature and inclusion time as factors， using the yield of inclusion compounds as index，the inclusion technology was optimized by orthogonal test. The formation of inclusion compound was identified by the spectra change of FT-IR，XRD and 1H NMR. Its structure was characterized by 1H ROESY NMR. RESULTS：The optimized inclusion conditions were that the molar ratio of eugenol to β-CD was 1.0 ∶1；inclusion temperature was 60 ℃；inclusion time was 2.0 h. And the yield of inclusion compound was 73.86％（RSD＝0.17％，n＝3）. 1H NMR results of β-CD and its inclusion complex indicated that the optimum qualitative ratio of the inclusion complex was 1.0 ∶1. The intermolecular interaction between eugenol and β-CD was confirmed by the spectrum analysis of FT-IR and XRD. 1H ROESY NMR results indicated the structure of inclusion complex mainly was that the phenyl of eugenol was in the cavity of β-CD，the vinyl was outside. CONCLUSIONS：The inclusion technology is reasonable and feasible，and can be used for the inclusion of eugenol and β-CD. The formation of inclusion compound is confirmed by the spectrum analysis.