OBJECTIVE：To establish a method for simultaneous determination of the confents of calycosin-7-glucoside，calycosin and formononetin in Shenqi granule. METHODS：HPLC was performed on the column of Welch Ultimate XB-C18 with mobile phase of acetonitrile-0.2％ Formic acid solution（gradient elution）at flow rate of 1.0 ml/min，detection wavelength was 250 nm， column temperature was 35 ℃，and injection volume was 10 μl. RESULTS：The linear range of sample quantity was 0.008-0.32 μg for calycosin-7-glucoside（r＝0.999 9），0.005-0.20 μg for calycosin（r＝0.999 7）and 0.007-0.28 μg for formononetin（r＝0.999 8）； RSDs of precsion，stability and reproducibility tests were no more than 1.59％；recoveries were 98.17％-99.17％（RSD＝0.67％， n＝6），98.03％-99.66％（RSD＝0.57％，n＝6）and 98.16％-99.12％（RSD＝0.43％，n＝6）. CONCLUSIONS：The method is simple， accurate and reliable，and can be used for the content determination of Shenqi granule ofiso flavones.