OBJECTIVE：To optimize the purification process of the extract from Yiqi guben granules. METHODS：The purification effect of the process was investigated with transfer rate of polysaccharide，calycosin glucoside and dry paste as evaluation indexes， using ZTC1 + 1 type Ⅱ，and shell poly sugar and 95％ ethanol as clarifying agents. The purification process of the extract from Yiqi guben granules was optimized by orthogonal test using the ratio of material to liquid，the amount of clarifying agent and standing time as factor. The validation test was conducted. RESULTS：Selecting ZTC1+1 type Ⅱ as a clarifying agent，the best transfer rate of effective component had been obtained；optimal purification process was as follows as the ratio of material to liquid 1 ∶2， the ratio of ZTC1 + 1 type ⅡA liquid 5％，the ratio of B liquid 10％，standing time of 5 h. The results of verification test showed transfer rates of dry paste in 3 tests were 71.54％ ，70.98％ ，69.21％ ，respectively；those of polysaccharide were 82.55％ ， 81.78％，82.15％，respectively；those of calycosin glucoside were 91.92％，92.34％，91.58％，respectively（all RSD≤1.72％， n＝3）. CONCLUSIONS：The optimized purification process is effective，stable and practical，and can be used for the purification of the extract from Yiqi guben granules.