OBJECTIVE：To investigate the association of Cytochrome P4503A family polypeptide 5 [（CYP）3A5] and IL-10 polymorphisms with the concentration/dose（C/D） ratio of tacrolimus in lung transplantation recipients. METHODS：CYP3A5 polymorphisms at rs774746 and IL-10 polymorphisms at rs1800896 from peripheral blood cell of 51 lung transplant recipients was detected by Sequenom MassArray iPLEX GOLD system. The relationship between gene polymorphism and tacrolimus C/D ratio was analyzed. RESULTS： The tacrolimus C/D ratio of the recipients who carried CYP3A5 rs776746 GG genotype were higher than those with AA+GG genotype，with statistical significance（P＜0.01）. The tacrolimus C/D ratio of the recipients who carried IL-10 rs1800896 AA genotype were higher than those with AG genotype during 1 week and 2 weeks after transplantation，with statistical significance（P＜0.05）. Combined analysis of these two genes，the tacrolimus C/D ratio of the recipients who carried more fast metabolic alleles were higher than those who didn’t carry fast metabolic alleles，with statistical significance（P＜0.01）. CONCLUSIONS：Determination of recipients CYP3A5 at rs 776746and IL-10 at rs800896 genotype may be performed prospectively to help individualizing tacrolimus dose regimen.