OBJECTIVE：To establish the quality standard for Yiwei granule. METHODS：TLC was conducted for the qualitative identification of Coptis chinensis，Corydalis yanhusuo and Schisandra chinensis；HPLC was conduced for the content determination of paeoniflorin. The column was XDB C18 with mobile phase of methanol-water（25 ∶75，V/V）at flow rate of 1.0 ml/min，detection wavelength was 230 nm and injection volume was 20 μl. RESULTS：The TLC showed clear spots and good separation. The linear range of paeoniflorin was 7.575-60.6 μg/ml（r＝0.999 9）；RSDs of precision，stability and reproducibility tests were lower than 2.0％，recovery was 96.97％-102.51％（RSD＝1.92％，n＝9）. CONCLUSIONS：The method is simple and reproducible，and can be used for the quality control of Yiwei granule.