OBJECTIVE：To establish a method to determine the content of chlorogenic acid in Yinhuang qinghuo capsule. METHODS：HPLC was performed on the column of Acclaim120? C18 with mobile phase of acetonitrile -0.4％ phosphoric acid（12 ∶ 88，V/V）at flow rate of 1 ml/min，the detection wavelength was 327 nm，column temperature was 25 ℃，and volume injection was 10 μl. RESULTS：The linear range of chlorogenic acid was 56-224 μg；RSDs of precision，stability and reproduciblity tests were lower than 3.0％，recovery was 96.91％-104.76％（RSD＝1.46％，n＝9）. CONCLUSIONS：The method is simple and reproducible， and can be used for the quality control of Yinhuang qinghuo capsule.