OBJECTIVE：To explore the effect of prophylactic use of vitamin B6 with chlorpheniramine on the adverse reactions in fundus fluorescein angiography （FFA）. METHODS：326 patients with FFA were randomly divided into observation group and control group. Observation group was orally given Vitamin B6 tablet 10 mg 30 min before angiography+Chlorpheniramine maleate tablet 4 mg； control group was orally given Metoclopramide tablet 10 mg+Chlorpheniramine maleate tablet 4 mg. The occurrence time and incidence of adverse reactions in 2 groups were observed and correlation analysis was conducted. RESULTS：There was no significant difference in the occurrence time of adverse reactions between 2 groups（P＞0.05）； the incidence of adverse reactions in observation group was significantly lower than control group， the difference was statistically significant（P＜0.05）. The correlation analysis of adverse reactions in control group showed the occurrence of adverse reactions had no correlation with age and gender of patients（P＞0.05）. CONCLUSIONS： Prophylactic use of vitamin B6 with chlorphenir arnine can reduce the occurrence of adverse reactions in FFA.