Study on the separation and identification of flavone <italic style="font-style: italic">C</italic>-glycosides from the leaves of <italic style="font-style: italic">Dendrobium officinale </italic>and their inhibitory activities to <italic style="font-style: italic">α</italic>-gulcosidase

FENG Hailin; PENG Chongsheng; LI Xiaobo

doi:10.6039/j.issn.1001-0408.2022.18.03

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Study on the separation and identification of flavone C-glycosides from the leaves of Dendrobium officinale and their inhibitory activities to α-gulcosidase

更新时间：2023-02-22

- Study on the separation and identification of flavone C-glycosides from the leaves of Dendrobium officinale and their inhibitory activities to α-gulcosidase
- ZHONGGUO YAOFANG Vol. 33, Issue 18, Pages: 2187-2191(2022)
- 作者机构：
  
  上海交通大学药学院，上海　200240
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.18.03
  CLC： R917
- Published：30 September 2022，
  
  Received：05 April 2022，
  
  Revised：05 June 2022，
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冯海林,彭崇胜,李晓波.铁皮石斛叶中黄酮碳苷类成分分离、鉴定及抑制α-葡萄糖苷酶活性研究 ^Δ[J].中国药房,2022,33(18):2187-2191.

FENG Hailin,PENG Chongsheng,LI Xiaobo.Study on the separation and identification of flavone C-glycosides from the leaves of Dendrobium officinale and their inhibitory activities to α-gulcosidase[J].ZHONGGUO YAOFANG,2022,33(18):2187-2191.
冯海林,彭崇胜,李晓波.铁皮石斛叶中黄酮碳苷类成分分离、鉴定及抑制α-葡萄糖苷酶活性研究 ^Δ[J].中国药房,2022,33(18):2187-2191. DOI： 10.6039/j.issn.1001-0408.2022.18.03.

FENG Hailin,PENG Chongsheng,LI Xiaobo.Study on the separation and identification of flavone C-glycosides from the leaves of Dendrobium officinale and their inhibitory activities to α-gulcosidase[J].ZHONGGUO YAOFANG,2022,33(18):2187-2191. DOI： 10.6039/j.issn.1001-0408.2022.18.03.

摘要

目的

分离、鉴定铁皮石斛叶中的黄酮碳苷类成分，并评价其体外抑制

-葡萄糖苷酶的活性。

方法

采用大孔吸附树脂和制备型高效液相色谱法对铁皮石斛叶中的黄酮碳苷类成分进行分离和纯化，通过紫外光谱、核磁共振、高分辨电喷雾离子化质谱等波谱方法对化合物进行推导和鉴定。考察黄酮碳苷类成分与阳性对照（阿卡波糖）的

-葡萄糖苷酶体外抑制活性。

结果

从铁皮石斛叶中分离得到5个芹菜素-6，8-

-二糖苷，鉴定为芹菜素-6

-C-α

-L-鼠李糖-8

-C-β

-D-异鼠李糖（

）、夏佛塔苷（

）、异夏佛塔苷（

）、异佛莱心苷（

）和佛莱心苷（

）。化合物

～

和阿卡波糖抑制

-葡萄糖苷酶的半数抑制浓度分别为（1.79±1.27）、（2.05±0.72）、（1.93±0.67）、（1.09±0.46）、（1.36±0.58）、（18.69±1.24）μmol/L。

结论

从铁皮石斛叶中分离得到5个具有

-葡萄糖苷酶抑制活性的芹菜素-6，8-

-二糖苷类成分，其中化合物

为新化合物，化合物

首次从该植物中分离得到。

Abstract

OBJECTIVE

To separate and identify the flavone

-glycosides from the leaves of

Dendrobium officinale

， and to evaluate their

in vitro

inhibitory activities to α-glucosidase.

METHODS

The flavone

-glycosides from the leaves of

D. officinale

were separated and purified by macroporous adsorption resin and preparative high-performance liquid chromatography. The structure of obtained compound was elucidated and identified by spectroscopic methods， such as ultraviolet spectrum， nuclear magnetic resonance， high-resolution electrospray ionization mass spectrometry， etc. The

in vitro

inhibitory activities of flavone

-glycosides and positive control （acarbose） to

-glucosidase were investigated.

RESULTS

Five apigenin-6，8-di-

-glycosides were isolated and purified from the leaves of

D. officinale

， and identified as apigenin-6-

-L-rhamnosyl-8-

-D-quinovoside（

）， schaftoside （

），isoschaftoside（

），isoviolanthin（

） and violanthin（

）. Half inhibitory concentration of compound

and acarbose inhibiting

-glucosidase were （1.79±1.27），（2.05±0.72），（1.93±0.67），（1.09±0.46），（1.36±0.58），（18.69±1.24） μmol/L， respectively.

CONCLUSIONS

Five apigenin-6，8-di-

-glycosides with

-glucosidase inhibitory activity are isolated from the leaves of

D. officinale

， of which compound

is a new compound and compound

is isolated from this plant for the first time.

关键词

铁皮石斛叶黄酮碳苷类芹菜素-6-C-α-L-鼠李糖-8-C-β-D-异鼠李糖α-葡萄糖苷酶

Keywords

the leaves of Dendrobium officinaleflavone C-glycosideapigenin-6-C-α-L-rhamnosyl-8-C-β-D-quinovosideα-gulcosidase

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Study on the separation and identification of flavone C-glycosides from the leaves of Dendrobium officinale and their inhibitory activities to α-gulcosidase

DOI：10.6039/j.issn.1001-0408.2022.18.03

摘要

Abstract

关键词

Keywords

references