Effects of <italic style="font-style: italic">Achyranthis bidentatae-Cynanchum otophyllum </italic>on oxidative stress of cerebral tissue in Parkinson’s disease mice with syndrome of hyperactivity of liver-Yang

RONG Hua; ZHANG Jingyan; LIU Jing; ZHANG Xiaojie

doi:10.6039/j.issn.1001-0408.2022.18.04

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Effects of Achyranthis bidentatae-Cynanchum otophyllum on oxidative stress of cerebral tissue in Parkinson’s disease mice with syndrome of hyperactivity of liver-Yang

更新时间：2023-02-22

- Effects of Achyranthis bidentatae-Cynanchum otophyllum on oxidative stress of cerebral tissue in Parkinson’s disease mice with syndrome of hyperactivity of liver-Yang
- ZHONGGUO YAOFANG Vol. 33, Issue 18, Pages: 2192-2197(2022)
- 作者机构：
  
  1.黑龙江中医药大学基础医学院，哈尔滨　150040
  2.齐齐哈尔医学院病理学院，黑龙江齐齐哈尔　161006
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.18.04
  CLC： R965
- Published：30 September 2022，
  
  Received：23 April 2022，
  
  Revised：12 August 2022，
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荣华,张静艳,刘京等.牛膝-白芍对肝阳上亢型帕金森病小鼠脑组织氧化应激的影响 ^Δ[J].中国药房,2022,33(18):2192-2197.

RONG Hua,ZHANG Jingyan,LIU Jing,et al.Effects of Achyranthis bidentatae-Cynanchum otophyllum on oxidative stress of cerebral tissue in Parkinson’s disease mice with syndrome of hyperactivity of liver-Yang[J].ZHONGGUO YAOFANG,2022,33(18):2192-2197.
荣华,张静艳,刘京等.牛膝-白芍对肝阳上亢型帕金森病小鼠脑组织氧化应激的影响 ^Δ[J].中国药房,2022,33(18):2192-2197. DOI： 10.6039/j.issn.1001-0408.2022.18.04.

RONG Hua,ZHANG Jingyan,LIU Jing,et al.Effects of Achyranthis bidentatae-Cynanchum otophyllum on oxidative stress of cerebral tissue in Parkinson’s disease mice with syndrome of hyperactivity of liver-Yang[J].ZHONGGUO YAOFANG,2022,33(18):2192-2197. DOI： 10.6039/j.issn.1001-0408.2022.18.04.

摘要

目的

探讨镇肝熄风汤的核心药对牛膝-白芍对肝阳上亢型帕金森病（PD）小鼠脑组织氧化应激的影响。

方法

将C57BL/6小鼠随机分为正常对照组、模型组、镇肝熄风汤组、牛膝组、白芍组、牛膝-白芍药对组，每组10只。采用附子煎液（4 g/kg）灌胃联合1-甲基-4苯基-1，2，3，6-四氢吡啶（25 mg/kg）腹腔注射方法复制肝阳上亢型PD小鼠模型。观察小鼠行为学变化和脑黑质神经元超微结构，检测小鼠脑黑质区酪氨酸羟化酶（TH）阳性神经元表达水平和脑黑质中总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、丙二醛（MDA）表达水平以及核因子E2相关因子2（Nrf2）、硫氧还蛋白还原酶1（Trxr1）、硫氧还蛋白互作蛋白（Txnip）mRNA和蛋白表达水平。

结果

与模型组比较，各给药组小鼠PD样行为和脑黑质神经元超微结构均有改善，TH阳性神经元表达水平，T-AOC（除白芍组外）、SOD（除白芍组外）表达水平以及Nrf2、Trxr1 mRNA相对表达量和蛋白表达水平均显著升高，MDA（除白芍组外）表达水平以及Txnip mRNA相对表达量和蛋白表达水平均显著降低（

＜0.05）。牛膝-白芍药对组的干预效果优于牛膝组和白芍组（

＜0.05），与镇肝熄风汤组作用相当（

＞0.05）。

结论

牛膝-白芍药对可能以Nrf2为靶点抑制肝阳上亢型PD小鼠脑组织氧化应激水平，对脑部神经元起到保护作用，效果优于牛膝、白芍单味药材，能够发挥与组方相当的补肾柔肝、潜阳熄风的功效。

Abstract

OBJECTIVE

To explore the effects of

Achyranthis bidentatae-Cynanchum otophyllum

as core couplet medicinals of Zhengan xifeng decoction on oxidative stress of cerebral tissue in Parkinson’s disease （PD） mice with syndrome of hyperactivity of liver-Yang.

METHODS

C57BL/6 mice were randomly divided into normal control group， model group， Zhengan xifeng decoction group，

A. bidntatae

group，

C. otophyllum

group and couplet medicinals of

A. bidentatae-C. otophyllum

group， with 10 mice in each group. PD model of hyperactivity of liver-Yang was established by intragastric administration of

Aconitum carmichaelii

decoction（4 g/kg） and intraperitoneal injection of 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine （25 mg/kg）.The behavioral changes and ultrastructure of substantia nigra neurons in mice were observed. The expressions of tyrosine hydroxylase （TH） positive neurons in substantia nigra were detected.The expressions of total antioxidant capacity （T-AOC），superoxide dismutase （SOD） and malondialdehyde （MDA） in substantia nigra as well as mRNA and protein expressions of nuclear factor-erythroid 2-related factor 2 （Nrf2）， thioredoxin reductase 1 （Trxr1）， thioredoxin interacting protein （Txnip） were also determined.

RESULTS

Compared with model group， PD behavior and ultrastructure of substantia nigra neurons were all improved in administration groups.The expressions of TH positive neurons， T-AOC （except for

C.otophyllum

group） and SOD （except for

C.otophyllum

group）， mRNA relative expression and protein expression levels of Nrf2 and Trxr1 were all increased significantly； the expression of MDA （except for

C.otophyllum

group） and mRNA relative expression and protein expression levels of Txnip were all decreased significantly （

＜0.05）. Intervention effect of couplet medicinals of

A. bidentatae-C. otophyllum

group was better than that of

A.bidntatae

group and

C. otophyllum

group （

＜0.05）， and the effect was similar to that of Zhengan xifeng decoction group （

＞0.05）.

CONCLUSIONS

The couplet medicinals of

A. bidentatae-C. otophyllum

can inhibit the level of oxidative stress in the cerebral tissue of PD mice with hyperactivity of liver-Yang by targeting Nrf2， and play a protective role on the brain neurons. Its effect is better than that of

A.bidntatae

and

C.otophyllum

， and it plays the same role as that of the formula in tonifying the kidney， softening the liver and suppressing the Yang.

关键词

帕金森病肝阳上亢牛膝白芍药对镇肝熄风汤氧化应激小鼠

Keywords

hyperactivity of liver-YangAchyranthis bidentataeCynanchum otophyllumcouplet medicinalsZhengan xifeng decoctionoxidative stressmice

references

PETRILLO S，SCHIRINZI T，DI LAZZARO G，et al. Systemic activation of Nrf2 pathway in Parkinson’s di-sease[J]. Mov Disord，2020，35（1）：180-184.

孙林娟，宁侠，周绍华.周绍华治疗帕金森病经验[J].中医杂志，2015，56（3）：193-197.

陈宏志，李静蔚，何建成. 基于临床病案文献的帕金森病中医基本证候研究[J]. 中华中医药杂志，2016，31（8）：3216-3219.

魏维，蔡晶.帕金森病的证型分布及中药治疗方药文献分析[J].中医杂志，2013，54（20）：1778-1782.

崔俊波. 镇肝熄风汤在内科疾病中的应用[J]. 河南中医，2014，34（2）：345-347.

顾超，袁灿兴.帕金森病的中医证型分布和用药规律探析[J].上海中药杂志，2013，47（7）：12-14.

LIU H，YU C L，XU T J，et al. Synergistic protective effect of paeoniflorin and β-ecdysterone against rotenone-induced neurotoxicity in PC12 cells[J]. Apoptosis，2016，21（12）：1354-1365.

兴桂华，王晓丽，丛欢，等.镇肝熄风汤对帕金森病模型小鼠神经行为学及黑质14-3-3、酪氨酸羟化酶表达的影响[J].中国实验方剂学杂志，2013，19（21）：190-193.

张静艳，郭科东，金明，等. 牛膝和白芍配伍对帕金森病肝阳上亢证小鼠的协同神经保护作用及机制研究[J]. 中南药学，2021，19（11）：2269-2275.

林臻，陈洪志，赵航，等. MPTP诱导C57BL/6小鼠帕金森模型的制备和评估[J]. 中国比较医学杂志，2020，30（8）：57-62，85.

李晓莉，杨斌，刘承伟. 帕金森病MPTP模型中老年小鼠两种行为学检测方法的比较研究[J]. 华夏医学，2013，26（2）：293-297.

BROOKS S P，DUNNETT S B. Tests to assess motor phenotype in mice：a user’s guide[J]. Nat Rev Neurosci，2009，10（7）：519-529.

王晓丽，孙影，朱兰芹，等. 镇肝熄风汤对帕金森病肝阳上亢证大鼠中脑Akt和GSK-3β磷酸化的影响[J]. 世界科学技术-中医药现代化，2016，18（8）：1374-1378.

杨雯晴，王晓冰，李运伦. 原发性高血压肝阳上亢证动物模型的评价与思考[J]. 山东中医杂志，2015，34（6）：403-405，429.

陈毅美，李琳，张彦芳，等. 京尼平苷对MPTP所致帕金森病小鼠模型的神经保护作用研究[J]. 神经解剖学杂志，2015，31（5）：629-634.

CENCI M A，CROSSMAN A R. Animal models of l-dopa-induced dyskinesia in Parkinson’s disease[J]. Mov Di-sord，2018，33（6）：889-899.

李亚平，施萌婧，臧运华. 浮针配合镇肝熄风汤治疗帕金森病及偏侧舞蹈症验案[J]. 实用中医药杂志，2022，38（2）：315-317.

田硕，苗明三. 牛膝的化学、药理及应用特点探讨[J]. 中医学报，2014，29（8）：1186-1188.

JOHNSON M E，LIM Y，SENTHILKUMARAN M，et al.Investigation of tyrosune hydroxylase and BDNF in a low-dose rotenoe model of Parkison’s disease[J].J Chen Neuroanat，2015，70（6）：33-41.

SINGH A，KUKRETI R，SASO L，et al. Oxidative stress：a key modulator in neurodegenerative diseases[J]. Mo-lecules，2019，24（8）：E1583.

BUENDIA I，MICHALSKA P，NAVARRO E，et al. Nrf2-ARE pathway：an emerging target against oxidative stress and neuroinflammation in neurodegenerative diseases[J]. Pharmacol Ther，2016，157：84-104.

NIU Y C，ZHANG J，DONG M X. Nrf2 as a potential target for Parkinson’s disease therapy[J]. J Mol Med（Berl），2021，99（7）：917-931.

YOSHUHARA E，MASJI S，MATSUO Y，et al.Thioredoxin/Txnip：redoxisome，as a redox switch for the pathogenesis of diseases[J].Front Immunol，2014，4：514.

莫与琳，杨亚军，崔燎. TXNIP介导的氧化应激在疾病中的作用机制[J]. 中国药理学通报，2018，34（1）：16-19.

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Effects of Achyranthis bidentatae-Cynanchum otophyllum on oxidative stress of cerebral tissue in Parkinson’s disease mice with syndrome of hyperactivity of liver-Yang

DOI：10.6039/j.issn.1001-0408.2022.18.04

摘要

Abstract

关键词

Keywords

references