OBJECTIVE

2

To establish the method for simultaneous determination of 11 components as narirutin in Biantong capsules， to conduct chemical pattern recognition analysis and to screen differential markers affecting their quality.

METHODS

2

HPLC method was adopted. The separation was carried out on Venusil XBP C

18

column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution with gradient elution at flow rate of 1.0 mL/min. The sample size was 10 µL， and column temperature was set at 30 ℃. The detection wavelengths were set at 283， 330， 520， 220 nm， respectively. Using verbascoside as an internal standard， the contents were determined by quantitative analysis of mult-components by single marker （QAMS）， and the results were compared with those of external standard method. Cluster analysis， principle component analysis and orthogonal partial least squares-discriminant analysis were performed with SPSS 26.0 and SIMCA 14.1 software. The differential markers affecting the quality of Biantong capsules were screened using the variable importance in projection （VIP） value greater than 1 as the standard.

RESULTS

2

The contents of narirutin， naringin， neohesperidin， echinacoside， tubuloside A， isoacteoside， cyanidin-3-

O

-glucoside， cyanidin-3-

O

-rutoside， atractylolide Ⅲand atractylolide Ⅰ were 0.739-1.265， 1.134-2.158， 1.407-2.359， 1.368-2.502， 0.304-0.522， 0.257-0.521， 0.423-0.727， 0.375-0.733， 0.130-0.283 and 0.062-0.166 mg/g， respectively. The relative average deviation of them from the external standard method was less than 2％. The results of cluster analysis showed that 15 batches of samples could be grouped into three categories， S1-S7 as a category， S8-S10 as a category， and S11-S15 as a category， which was consistent with the classification results of principal component analysis. The results of orthogonal partial least squares-discriminant analysis showed that the VIP values of cyanidin-3-

O

-rutoside， atractylolide Ⅲ， naringin， neohesperidin， echinacoside and verbascoside were all greater than 1.

CONCLUSIONS

2

The method for simultaneous determination of 11 components in Biantong capsules， including narirutin， is successfully established. Combined with chemical pattern recognition analysis， it can be used for the quality control of Biantong capsules. Six components such as cyanidin-3-

O

-rutoside may be the differential markers that affect the quality of Biantong capsules.