OBJECTIVE

2

To study the intervention effects and mechanism of Compound yu’e nasal drops on ovalbumin induced allergic rhinitis in rats.

METHODS

2

The allergic rhinitis model of rat was induced with ovalbumin. Model rats were randomly divided into model group， triamcinolone acetonide group （positive control， 0.026 mg/kg）， Compound yu’e nasal drops high-dose， medium-dose and low-dose groups （134.4、67.2、33.6 mg/kg）， 12 rats in each group. Another blank control group was set. Except for blank control group， the corresponding drugs were given by nasal drip twice a day for 14 days. One hour after last administration， the nasal symptom scores of rats were recorded； the levels of serum immunoglobulin E （IgE）， interleukin-2 （IL-2）， IL-13 and tumor necrosis factor-α （TNF-α）were measured by enzyme-linked immunosorbent assay. The changes of nasal mucosa in rat were observed by HE staining. The expressions of TNF-α， IL-2 and IL-13 in nasal mucosa were detected by Western blot.

RESULTS

2

Compared with blank control group， nasal symptom score and the levels of serum IgE， IL-2， IL-13， TNF-α in model group were increased significantly （

P

＜0.01）； obvious pathological injury was found in nasal mucosa， and the expressions of TNF-α， IL-2 and IL-13 protein were increased significantly （

P

＜0.01）. Compared with model group， Compound yu’e nasal drops significantly reduced the nasal symptom score， the levels of serum IgE， IL-2， IL-13， TNF-α to different extents， improved pathological injury of nasal mucosa and significantly inhibited the expressions of TNF-α， IL-2 and IL-13 protein（

P

＜0.05 or

P

＜0.01）.

CONCLUSIONS

2

Compound yu’e nasal drops play significant effects against allergic rhinitis in rats by regulating the balance of type 1 helper T cells/type 2 helper T cells， balancing and inhibiting the secretion of inflammatory cytokines.