- Journal Information
- Archive
- Submission
- For Reviewers
- Statement and Policies
- 个人中心
- 退出登录
浏览全部资源
扫码关注微信
- Journal Information
- Journal Introduction
- Editorial Board
- Youth Editorial Board
- Editorial Board Charter
- Subscriptions
- Archive
- Current Issue
- Past Issues
- Priority Publication
- Virtual Collection
- Recommended Articles
- Submission
- Submission Process
- Submission Guidelines
- Download
- Contact us
- For Reviewers
- Guidelines for Reviewers
- Peer Reviewed Policies
- Statement and Policies
- Ethics and Malpractice
- Research Ethics Policies
- Repetition Detection
- Corrections and Retractions
- Editorial Policies
- Open Access Statement
- Website Responsibility
Effects of naringin on the proliferation and differentiation of osteoblasts co-cultured with T cells under simulated microgravity
- ZHONGGUO YAOFANG Vol. 33, Issue 19, Pages: 2333-2337(2022)
- 作者机构：
哈尔滨医科大学附属第二医院骨四科，哈尔滨 150086
- 作者简介：
- 基金信息：
DOI：10.6039/j.issn.1001-0408.2022.19.06
CLC： R965Received：25 May 2022，
Revised：2022-07-28，
Published：15 October 2022
- 稿件说明：
移动端阅览
陈建兴,尹文哲,孙奇峰等.模拟微重力下柚皮苷对T细胞共育的成骨细胞增殖分化的影响 Δ[J].中国药房,2022,33(19):2333-2337.
CHEN Jianxing,YIN Wenzhe,SUN Qifeng,et al.Effects of naringin on the proliferation and differentiation of osteoblasts co-cultured with T cells under simulated microgravity[J].ZHONGGUO YAOFANG,2022,33(19):2333-2337.
陈建兴,尹文哲,孙奇峰等.模拟微重力下柚皮苷对T细胞共育的成骨细胞增殖分化的影响 Δ[J].中国药房,2022,33(19):2333-2337. DOI： 10.6039/j.issn.1001-0408.2022.19.06.
CHEN Jianxing,YIN Wenzhe,SUN Qifeng,et al.Effects of naringin on the proliferation and differentiation of osteoblasts co-cultured with T cells under simulated microgravity[J].ZHONGGUO YAOFANG,2022,33(19):2333-2337. DOI： 10.6039/j.issn.1001-0408.2022.19.06.
摘要
目的
2
在模拟微重力情况下，探索柚皮苷作用于T细胞-成骨细胞共育体系后，对成骨细胞增殖分化的影响。
方法
2
本实验设正常重力对照组、正常重力柚皮苷组、模拟微重力对照组、模拟微重力柚皮苷组，其中对照组分别在正常重力或模拟微重力条件下将T细胞与成骨细胞共育，柚皮苷组分别在对照组基础上加入1×10
－5
mol/L柚皮苷溶液。显微镜下观察成骨细胞形态学，CCK-8法检测成骨细胞增殖率，碱性磷酸酶（ALP）试剂盒检测ALP活性，实时荧光定量聚合酶链式反应检测Runt相关转录因子2（Runx2）、白细胞介素6（IL-6）mRNA相对表达水平，Western blot法检测Runx2和IL-6蛋白相对表达水平。
结果
2
与正常重力对照组比较，模拟微重力对照组成骨细胞密度降低，仅有少量聚集，细胞形态以圆形居多；与模拟微重力对照组比较，模拟微重力柚皮苷组成骨细胞成梭形或多角形，形态饱满，成鱼群状聚集。与正常重力对照组比较，正常重力柚皮苷组成骨细胞增殖率、ALP活性、Runx2 mRNA和蛋白相对表达水平显著升高，IL-6 mRNA和蛋白相对表达水平显著降低（
P
＜0.05）；模拟微重力对照组成骨细胞增殖率、ALP活性、Runx2 mRNA和蛋白相对表达水平显著降低，IL-6 mRNA和蛋白相对表达水平显著升高（
P
＜0.05）。与模拟微重力对照组比较，模拟微重力柚皮苷组成骨细胞增殖率、ALP活性、Runx2 mRNA和蛋白相对表达水平显著升高，IL-6 mRNA和蛋白相对表达
水平显著降低（
P
＜0.05）。
结论
2
在T细胞-成骨细胞共育体系中，模拟微重力可以抑制成骨细胞的增殖分化能力；该条件下柚皮苷可以改善成骨细胞的增殖分化能力，其作用机制与IL-6水平的降低及Runx2水平的升高有关。
Abstract
OBJECTIVE
2
To explore the effects of naringin on the proliferation and differentiation of osteoblasts in T cell-osteoblast co-culture system under simulated microgravity.
METHODS
2
This experiment was setting normal gravity control group， normal gravity naringin group， simulated microgravity control group and simulated microgravity naringin group.In the control group， T cells were co-cultured with osteoblasts under normal gravity or simulated microgravity conditions. In the naringin group， 1×10
－5
mol/L naringin solution was added additionally on the basis of control group.The morphology of osteoblasts was observed under the microscope. The proliferation rate of osteoblasts was detected by CCK-8 method. The alkaline phosphatase （ALP） activity was detected by ALP kit. mRNA relative expressions of Runt related transcription factor 2 （Runx2） and interleukin-6 （IL-6） were detected by real-time fluorescence quantitative polymerase chain reaction. The relative expression of Runx2 and IL-6 protein were detected by Western blot.
RESULTS
2
Compared with normal gravity control group， the density of osteoblasts in the simulated microgravity control group decreased， only a small number of cells were aggregated， and the cell morphology was mostly round； compared with the simulated microgravity control group， the osteoblasts in the simulated microgravity naringin group formed spindle or polygonal， plump in shape， and clustered in groups of fish. Compared with normal gravity control group， the proliferation rate of osteoblasts， ALP activity， relative expressions of Runx2 mRNA and protein in the normal gravity naringin group were significantly increased， while the relative expressions of IL-6 mRNA and protein wer
e significantly decreased （
P
＜0.05）； the proliferation rate of osteoblasts， ALP activity， relative expressions of Runx2 mRNA and protein in the simulated microgravity control group were significantly decreased， while relative expressions of IL-6 mRNA and protein were significantly increased （
P
＜0.05）. Compared with the simulated microgravity control group， the proliferation rate of osteoblasts， ALP activity， relative expressions of Runx2 mRNA and protein in the simulated microgravity naringin group were significantly increased， while relative expressions of IL-6 mRNA and protein were significantly decreased （
P
＜0.05）.
CONCLUSIONS
2
In the T cell-osteoblast co-culture system， simulated microgravity can inhibit the proliferation and differentiation of osteoblasts；naringin can improve the proliferation and differentiation ability of osteoblasts under this condition， and its mechanism of action is related to the decrease of IL-6 level and the increase of Runx2 level.
关键词
Keywords
references
KOZLOVSKAYAI B ， GRIGORIEVA I . Russian system of countermeasures on board of the International Space Station （ISS）：the first results [J ] . Acta Astronaut ， 2004 ， 55 （ 3/4/5/6/7/8/9 ）： 233 - 237 .
MORRISON S J ， SCADDEN D T . The bone marrow niche for haematopoietic stem cells [J ] . Nature ， 2014 ， 505 （ 7483 ）： 327 - 334 .
CRUCIAN B ， STOWE R ， MEHTA S ， et al . Immune system dysregulation occurs during short duration spaceflight on board the space shuttle [J ] . J Clin Immunol ， 2013 ， 33 （ 2 ）： 456 - 465 .
VICO L ， COLLET P ， GUIGNANDON A ， et al . Effects of long-term microgravity exposure on cancellous and cortical weight-bearing bones of cosmonauts [J ] . Lancet ， 2000 ， 355 （ 9215 ）： 1607 - 1611 .
LAU R Y C ， GUO X . A review on current osteoporosis research：with special focus on disuse bone loss [J ] . J Osteoporos ， 2011 ， 2011 ： 293808 .
秦岭 ， 石印玉 ， “倡议”工作组 . 建立防治骨质疏松症的中草药研发国际标准的倡议 [J ] . 中国骨质疏松杂志 ， 2004 ， 10 （ 3 ）： 356 - 361，366 .
尹文哲 ， 张小玲 ， 叶义杰 ， 等 . 骨碎补对微重力下共育骨细胞中成骨细胞分化的影响 [J ] . 中医药学报 ， 2017 ， 45 （ 4 ）： 16 - 20 .
LI G L ， XU Y R ， SHENG X ， et al . Naringin protects against high glucose-induced human endothelial cell injury via antioxidation and CX3CL1 downregulation [J ] . Cell Physiol Biochem ， 2017 ， 42 （ 6 ）： 2540 - 2551 .
YU M F ， YOU D Q ， ZHUANG J J ， et al . Controlled release of naringin in metal-organic framework-loaded mine- ralized collagen coating to simultaneously enhance osseoin- tegration and antibacterial activity [J ] . ACS Appl Mater Interfaces ， 2017 ， 9 （ 23 ）： 19698 - 19705 .
WONG R W ， RABIE A B . Effect of naringin on bone cells [J ] . J Orthop Res ， 2006 ， 24 （ 11 ）： 2045 - 2050 .
ZHOU X X ， ZHANG P ， ZHANG C ， et al . Promotion of bone formation by naringin in a titanium particle-induced diabetic murine calvarial osteolysis model [J ] . J Orthop Res ， 2010 ， 28 （ 4 ）： 451 - 456 .
KANDHARE A D ， GHOSH P ， BODHANKAR S L . Naringin，a flavanone glycoside，promotes angiogenesis and inhibits endothelial apoptosis through modulation of inflammatory and growth factor expression in diabetic foot ulcer in rats [J ] . Chem Biol Interact ， 2014 ， 219 ： 101 - 112 .
WUJ B ， FONGY C ， TSAIH Y ， et al . Naringin-induced bone morphogenetic protein-2 expression via PI3K，Akt，c-Fos/c-Jun and AP-1 pathway in osteoblasts [J ] . Eur J Pharmacol ， 2008 ， 588 （ 2/3 ）： 333 - 341 .
MORABITO C ， GUARNIERI S ， CUCINA A ， et al . Antioxidant strategy to prevent simulated microgravity-induced effects on bone osteoblasts [J ] . Int J Mol Sci ， 2020 ， 21 （ 10 ）： 3638 .
DANKS L ， TAKAYANAGI H . Immunology and bone [J ] . J Biochem ， 2013 ， 154 （ 1 ）： 29 - 39 .
ROBINSON J W ， LI J Y ， WALKER L D ， et al . T cell-expressed CD40L potentiates the bone anabolic activity of intermittent PTH treatment [J ] . J Bone Miner Res ， 2015 ， 30 （ 4 ）： 695 - 705 .
JIMI E ， TAKAKURA N ， HIURA F ， et al . The role of NF-κB in physiological bone development and inflammatory bone diseases：is NF-κB inhibition “killing two birds with one stone” ? [J ] . Cells ， 2019 ， 8 （ 12 ）： 1636 .
HOFBAUERL C ， SCHOPPET M . Clinical implications of the osteoprotegerin/RANKL/RANK system for bone and vascular diseases [J ] . JAMA ， 2004 ， 292 （ 4 ）： 490 - 495 .
TAKAYANAGI H ， OGASAWARA K ， HIDA S ， et al . T-cell-mediated regulation of osteoclastogenesis by signalling cross-talk between RANKL and IFN-gamma [J ] . Nature ， 2000 ， 408 （ 6812 ）： 600 - 605 .
CHANG J ， WANG Z ， TANG E ， et al . Inhibition of osteoblastic bone formation by nuclear factor-kappaB [J ] . Nat Med ， 2009 ， 15 （ 6 ）： 682 - 689 .
韩标 ， 张扬 ， 李昊 ， 等 . 模拟微重力环境下核因子κB信号通路调节MC3T3-E1细胞成骨分化的实验研究 [J ] . 生物医学工程学杂志 ， 2019 ， 36 （ 3 ）： 421 - 427 .
SONG S H ， GAO Z Y ， LEI X J ， et al . Total flavonoids of drynariae rhizoma prevent bone loss induced by hindlimb unloading in rats [J ] . Molecules ， 2017 ， 22 （ 7 ）： E1033 .
任静 ， 青薇 ， 郑佳俊 ， 等 . 新型抗菌肽DP7对大鼠骨髓间充质干细胞影响探讨 [J ] . 实用医院临床杂志 ， 2020 ， 17 （ 1 ）： 1 - 6 .
付殷 ， 孙贵才 ， 陈水林 ， 等 . 基于雌激素受体研究氯化锂干预成骨细胞的分化及自噬 [J ] . 中国组织工程研究 ， 2019 ， 23 （ 5 ）： 680 - 684 .
周骅 ， 曹新生 ， 胡泽兵 ， 等 . 模拟微重力通过调节AKT磷酸化抑制前成骨细胞的增殖 [J ] . 现代生物医学进展 ， 2017 ， 17 （ 2 ）： 229 - 232 .
YANG C ， LIU W ， SHAN H J ， et al . Naringin inhibits titanium particles-induced up-regulation of TNF-α and IL-6 via the p38 MAPK pathway in fibroblasts from hip periprosthetic membrane [J ] . Connect Tissue Res ， 2021 ， 62 （ 5 ）： 485 - 494 .
0
Views
4
下载量
0
CSCD
- L-PDFDownload
- WORDDownload
- Meta-XMLDownload
- BibTeXDownload
- EndnoteDownload
- RefMan Download
- NoteExpressDownload
- NoteFirstDownload
Publicity Resources
Related Articles
Related Author
Related Institution
- Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
- Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号
公网安备50010302001817 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.