OBJECTIVE

2

To study the protective effect of phenolic acid components from

Salvia deserta

Schang on the oxidative stress injury of human renal tubular epithelial cells HK-2 induced by high glucose and high fat.

METHODS

2

HK-2 cells were divided into control group， model group， canagliflozin group （positive control group， 15 μmol/L）， purified product of phenolic acids from

S. deserta

Schang group （10.8 μg/mL）， 4 monomers group （salvianic acid， protocatechuic aldehyde， caffeic acid， rosmarinic acid， 50 μmol/L）. In addition to the control group， cell injury model of high glucose and high fat was established in other groups （500 μmol/L palmitic acid+30 mmol/L glucose for 48 h） and cultured for 48 h. The cell apoptotic rate， the contents of malondialdehyde （MDA） and glutathione （GSH）， and the activity of superoxide dismutase （SOD） were detected in each group； the expression levels of nuclear erythroid 2-related factor 2 （Nrf2），Kelch-like ECH-associated protein 1 （Keap1） protein， heme oxygenase-1（HO-1） and NADH： quinone acceptor oxidoreductase 1 （NQO1） were determined in above 5 groups （except for salvianic acid， protocatechuic aldehyde， caffeic acid）.

RESULTS

2

Compared with control group， the apoptotic rate of HK-2 cells in model group was increased significantly （

P

＜0.01）； the content of MDA was increased significantly （

P

＜0.01），while the content of GSH and the activity of SOD were decreased significantly （

P

＜0.01）； protein

expressions of Nrf2， NQO1 and HO-1 were significantly down-regulated （

P

＜0.01），while the protein expression of Keap1 was up-regulated significantly （

P

＜0.01）. Compared with model group， the apoptotic rate and the content of MDA were decreased significantly in administration groups （

P

＜0.01）； the content of GSH in administration groups and the activity of SOD in purified product of phenolic acids group， protocatechuic aldehyde group and rosmarinic acid group were increased significantly （

P

＜0.01）. The protein expressions of Nrf2， HO-1 and NQO1 in purified product of phenolic acids group as well as the protein expression of Nrf2 in rosmarinic acid group were up-regulated significantly （

P

＜0.01）， while the protein expression of Keap1 was down-regulated significantly in purified product of phenolic acids group and rosmarinic acid group （

P

＜0.01）.

CONCLUSIONS

2

The phenolic acids components from

S. deserta

Schang can relieve oxidative stress injury of renal tubular epithelial cells induced by high glucose and high fat， the mechanism of which may be associated with activating Keap1/Nrf2 signaling pathway and inhibiting oxidative stress response.