Simultaneous determination of 5 kinds of pentacyclic triterpenoids in <italic style="font-style: italic">Chaenomeles speciosa</italic> by quantitative analysis of multi-components by single-marker

ZHANG Tingting; HU Haoning; LI Pingyuan; HUANG Yongmei; WANG Junzhi; TANG Haiming; YIN Yonghong

doi:10.6039/j.issn.1001-0408.2022.20.09

您当前的位置：

首页 >

文章列表页 >

Simultaneous determination of 5 kinds of pentacyclic triterpenoids in Chaenomeles speciosa by quantitative analysis of multi-components by single-marker

更新时间：2025-12-05

- Simultaneous determination of 5 kinds of pentacyclic triterpenoids in Chaenomeles speciosa by quantitative analysis of multi-components by single-marker
- ZHONGGUO YAOFANG Vol. 33, Issue 20, Pages: 2477-2480(2022)
- 作者机构：
  
  1.三峡大学健康医学院，湖北宜昌　443002
  2.三峡大学生物与制药学院，湖北宜昌　443002
  3.湖北恒安芙林药业股份有限公司，湖北宜昌　443103
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.20.09
  CLC： R917
- Received：12 April 2022，
  
  Revised：2022-09-11，
  
  Published：30 October 2022
- 稿件说明：
移动端阅览
张亭亭,胡浩宁,李平媛,等.一测多评法同时测定木瓜中5种五环三萜类成分的含量[J].中国药房,2022,33(20):2477-2480.

ZHANG Tingting,HU Haoning,LI Pingyuan,et al.Simultaneous determination of 5 kinds of pentacyclic triterpenoids in Chaenomeles speciosa by quantitative analysis of multi-components by single-marker[J].ZHONGGUO YAOFANG,2022,33(20):2477-2480.
张亭亭,胡浩宁,李平媛,等.一测多评法同时测定木瓜中5种五环三萜类成分的含量[J].中国药房,2022,33(20):2477-2480. DOI： 10.6039/j.issn.1001-0408.2022.20.09.

ZHANG Tingting,HU Haoning,LI Pingyuan,et al.Simultaneous determination of 5 kinds of pentacyclic triterpenoids in Chaenomeles speciosa by quantitative analysis of multi-components by single-marker[J].ZHONGGUO YAOFANG,2022,33(20):2477-2480. DOI： 10.6039/j.issn.1001-0408.2022.20.09.

摘要

目的

建立同时测定木瓜中3-

-乙酰坡模醇酸等5种五环三萜类成分含量的方法，并分析不同产地、不同炮制工艺木瓜的含量差异。

方法

以高效液相色谱法为检测手段，色谱柱为COSMOSIL 5 C

-MS-Ⅱ，流动相为乙腈-0.005 mol/L磷酸氢二铵溶液（用磷酸调pH至6.5）（70︰30，

），流速为1.0 mL/min，柱温为30 ℃，检测波长为210 nm，进样量为20 μL。以齐墩果酸为内参物，结合一测多评法，对3-

-乙酰坡模醇酸等4种五环三萜类成分含量进行测定，并与外标法进行比较；同时，对不同产地、不同炮制加工工艺木瓜中齐墩果酸与熊果酸的总含量和5种成分的总含量进行比较。

结果

-乙酰坡模醇酸、白桦脂酸、齐墩果酸、熊果酸、3-

-乙酰熊果酸检测质量浓度的线性范围分别为4.06～81.2、2.12～42.4、9.62～192.3、7.77～155.4、4.21～84.1 μg/mL（

＞0.999）；精密度、重复性、稳定性（24 h）试验的RSD均小于3%；平均加样回收率为98.29%～101.38%（RSD＜3%，

＝6）。一测多评法测得3-

-乙酰坡模醇酸、白桦脂酸、熊果酸、3-

-乙酰熊果酸的含量分别为0.023%～0.060%、0.044%～0.528%、0.101%～0.368%、0.067%～0.221%，与外标法所测结果的相对偏差均小于8%。同一产地鲜切工艺加工木瓜中齐墩果酸与熊果酸的总含量和5种成分的总含量均高于传统工艺加工木瓜，且重庆黔江产木瓜中5种成分的总含量显著高于其他产地（

＜0.05）。

结论

建立了同时测定木瓜中3-

-乙酰坡模醇酸、白桦脂酸、齐墩果酸、熊果酸、3-

-乙酰熊果酸含量的一测多评法，该方法简便、快速、准确。重庆黔江产木瓜中5种成分的总含量较高，且同一产地鲜切工艺加工木瓜总含量高于传统工艺加工木瓜。

Abstract

OBJECTIVE

To establish a method for simultaneous determination of 5 kinds of pentacyclic triterpenoids as 3-

-acetyl-pomolic acid in

Chaenomeles speciosa

， and to analyze the difference in the contents of

C. speciosa

fro

m different producing areas by different processing technologies.

METHODS

HPLC method was adopted. The determination was performed on COSMOSIL 5 C

-MS-Ⅱ column with mobile phase consisted of acetonitrile-0.005 mol/L ammonium dihydrogen phosphate solution （pH value adjusted to 6.5 with phosphoric acid）（70∶30，

） at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃. The detection wavelength was set at 210 nm， and sample size was 20 μL. The contents of the other four pentacyclic triterpenoids were calculated according to quantitative analysis of multi-components by single-marker （QAMS） using oleanolic acid as internal reference. The results were compared with those determined by external standard method. The total content of oleanolic acid and ursolic acid， the total content of 5 components in

C. speciosa

from different producing areas and different processing technologies were compared.

RESULTS

The linear range of 3-

-acetyl-pomolic acid， betulinic acid， oleanolic acid， ursolic acid and 3-

-acetyl ursolic acid were 4.06-81.2， 2.12-42.4， 9.62-192.3， 7.77-155.4， 4.21-84.1 μg/mL， respectively （

＞0.999）. RSDs of precision， reproducibility and stability （24 h） tests were all lower than 3%. The average recoveries were 98.29%-101.38%（RSDs＜3%，

＝6）. The mass fraction of 3-

-acetyl-pomolic acid， betulinic acid， ursolic acid and 3-

-acetyl ursolic acid measured by QAMS were 0.023%-0.060%， 0.044%-0.528%， 0.101%-0.368%， 0.067%-0.221%， respectively； the deviations from the results measured by external standard method were all within 8%. The total content of oleanolic acid and ursolic acid， the total content of 5 components in

C. speciosa

processed by fresh-cut technology from the same producing area were higher than those in

C. speciosa

processed by traditional technology， and the total content of 5 components in

C. speciosa

from Chongqing Qianjiang were significantly higher than those from other producing areas （

＜0.05）.

CONCLUSIONS

QAMS method is established for the simultaneous determination of 3-

-acetyl-pomolic acid， betulinic acid， oleanolic acid， ursolic acid and 3-

-acetyl ursolic acid in

C. speciosa.

Established method is simple， rapid and accurate. The total content of 5 components in

C. speciosa

produced in Chongqing Qianjiang is higher， and the total content of

C. speciosa

processed by fresh-cut technology from the same origin is higher than

C. speciosa

　processed by traditional technology.

关键词

Keywords

references

唐春红，叶志义，项昭保，等 . 木瓜营养保健作用研究动态 [J ] . 天然产物研究与开发， 2000 ， 12 （ 4 ）： 97 - 100 .

肖苏龙，王晗，王琪，等 . 基于五环三萜先导结构的抗病毒抑制剂研究进展 [J ] . 中国科学：化学， 2015 ， 45 （ 9 ）： 865 - 883 .

王艺玮，徐盛涛，徐进宜 . 五环三萜羽扇豆烷型天然产物抗肿瘤活性研究进展 [J ] . 药学与临床研究， 2017 ， 25 （ 4 ）： 336 - 342，347 .

国家药典委员会 . 中华人民共和国药典：一部 [S ] . 2020年版. 北京：中国医药科技出版社， 2020 ： 62 .

王智民，钱忠直，张启伟，等 . 一测多评法建立的技术指南 [J ] . 中国中药杂志， 2011 ， 36 （ 6 ）： 657 - 658 .

卿大双，罗维早，孙建彬，等 . 一测多评法测定黄连及其炮制品中6种生物碱 [J ] . 中草药， 2016 ， 47 （ 2 ）： 324 - 329 .

陈俊，许浚，张静雅，等 . 基于一测多评法对延胡索中生物碱类成分的质量控制研究 [J ] . 中草药， 2016 ， 47 （ 3 ）： 493 - 498 .

张勇，张玲，谢晓梅 . 一测多评法同时测定木瓜中2种常见三萜酸 [J ] . 中成药， 2013 ， 35 （ 4 ）： 770 - 773 .

冯旻璐，许海燕，贺海波，等 . 木瓜总三萜通过调节miR-10a和PI3K/Akt/mTOR/p70S6K信号通路诱导胃癌细胞凋亡 [J ] . 中药材， 2019 ， 42 （ 12 ）： 2929 - 2935 .

贺海波，李小琴，李小妹，等 . 木瓜总三萜和奥美拉唑联用对吲哚美辛诱导大鼠胃溃疡的治疗作用研究 [J ] . 中国中药杂志， 2019 ， 44 （ 11 ）： 2338 - 2347 .

KAKUNO T ， YOSHIKAWA K ， ARIHARA S . Triterpenoid saponins from Ilex crenata fruit [J ] . Phytochemistry ， 1992 ， 31 （ 10 ）： 3553 - 3557 .

TALAPATRA S K ， SARKAR A C ， TALAPATRA B . Two pentacyclic triterpenes from Rubia cordifolia [J ] . Phytochemistry ， 1981 ， 20 （ 8 ）： 1923 - 1927 .

阚金涛，张清豫，袁雷，等 . 西藏皱皮木瓜中齐墩果酸、熊果酸提取工艺优化及不同干燥方式的含量分析 [J ] . 高原农业， 2019 ， 3 （ 3 ）： 300 - 307 .

蔡娟，刘世尧，韦正鑫，等 . 皱皮木瓜皮渣齐墩果酸和熊果酸提取工艺优化研究 [J ] . 食品工业科技， 2015 ， 36 （ 2 ）： 282 - 285，290 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Quality evaluation of Xintong granules based on HPLC fingerprint and quantitative analysis of multi-components by single-marker method

Study on fingerprint establishment， content determination， and spectrum-effect relationship of in vitro antioxidant activity for Bushen ningshen ointment

Establishment of quantitative analysis of multi-components by single-marker method for content determination of flavonoids and phenolic acids in Perilla frutescens leaves

Identification of tortoiseshell glue and antler glue in Qixiong zuogui granules and content determination of 12 chemical components

Establishment of HPLC fingerprint and content determination of multiple index components in Xiao’er resuqing oral liquid

Related Author

FENG Xiaolong

SHAO Mingguo

WAN Linchun

HU Zhenyu

CHEN Chunyu

WU Yu

BU Junwen

QIAN Yuhang

Related Institution

Jiangxi Institute for Drug Control

Lunan Pharmaceutical Group Co.， Ltd.

School of Pharmacy， Jiangxi University of Chinese Medicine

Key Laboratory of TCM Clinical Pharmacy， Shenzhen Bao'an Authentic TCM Therapy Hospital

Dept. of Preventive Treatment of Disease， Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine

Address：8th Floor, Sihuan Building, No. 129 Daping Main Street, Yuzhong District, Chongqing, China Postal code：400042
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Simultaneous determination of 5 kinds of pentacyclic triterpenoids in Chaenomeles speciosa by quantitative analysis of multi-components by single-marker

DOI：10.6039/j.issn.1001-0408.2022.20.09

摘要

Abstract

关键词

Keywords

references