OBJECTIVE

2

To establish a fingerprint and quantitative method of multi-components for Qianyang yuyin granules.

METHODS

2

Using the peak of loganin as reference， fingerprints of 15 batches of Qianyang yuyin granules were established by ultra-high liquid chromatography （UPLC）. The common peaks were identified and the similarity analysis was performed by

Similarity Evaluation System of Chromatographic Fingerprint of TCM

（2

004A edition）. Cluster analysis （CA）， principal component analysis （PCA） and partial least square-discriminant analysis （PLS-DA） were performed， and the differential components affecting the quality of Qianyang yuyin granules were screened by the criterion of the value of variable importance in the projection （VIP）＞1.0. UPLC was used to determine the contents of the differential components and harpagide， ferulic acid， cyasterone.

RESULTS

2

A total of 18 common peaks were identified from the fingerprints of 15 batches of samples， and the similarities were all higher than 0.96. It was identified that peak 6 was morroniside， peak 7 was loganin， peak 11 was hyperoside， peak 12 was stilbene glycoside， peak 13 was ferulic acid， peak 14 was cyasterone， peak 16 was harpagoside， peak 17 was cinnamic acid， and peak 18 was emodin. The results of CA and PCA showed that the 15 batches of samples could be divided into 6 categories. S1， S2， S4-S6 were grouped into one category， S3 was grouped into one category， S7-S8 were grouped into one category， S9-S10 were grouped into one category， S11-S14 were grouped into one category， S15 was grouped into one category. VIP values of peak 3， 9， 6 （morroniside）， 7 （loganin）， 15， 12 （stilbene glycoside） and 16 （harpagoside） were greater than 1.0. Among 15 batches of samples， the contents of harpagide， harpagoside， morroniside， loganin， stilbene glycoside， ferulic acid and cyasterone were 0.336-0.563， 0.205-0.377， 0.692-0.837， 0.433-0.562， 1.294-2.030， 0.020-0.027， 0.167-0.235 mg/g， respectively.

CONCLUSIONS

2

The UPLC fingerprint and multi-components quantitative analysis of Qianyang yuyin granules are successfully established， which can be used to improve the quality control standard of the preparation；morroniside， loganin， stilbene glycoside， harpagoside and other components could be the differential components affecting the quality of this preparation.