Study on the effects of Jinbei oral liquid against influenza A H1N1 virus and secondary <italic style="font-style: italic">Streptococcus pneumoniae</italic> infection of mice

ZHAO Fangshu; ZHANG Aijun; LIU Miaomiao; TIAN Jingzhen; HOU Lin

doi:10.6039/j.issn.1001-0408.2022.21.11

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Study on the effects of Jinbei oral liquid against influenza A H1N1 virus and secondary Streptococcus pneumoniae infection of mice

更新时间：2023-02-22

- Study on the effects of Jinbei oral liquid against influenza A H1N1 virus and secondary Streptococcus pneumoniae infection of mice
- ZHONGGUO YAOFANG Vol. 33, Issue 21, Pages: 2622-2626(2022)
- 作者机构：
  
  1.山东中医药大学药学院，济南　250355
  2.山东宏济堂制药集团股份有限公司，济南　250109
  3.山东中医药大学青岛中医药科学院，山东青岛　266112
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.21.11
  CLC： R965;R285
- Published：15 November 2022，
  
  Received：06 June 2022，
  
  Revised：10 October 2022，
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赵方舒,张爱均,刘苗苗等.金贝口服液抗小鼠甲型H1N1流感病毒及继发性肺炎链球菌感染作用研究 ^Δ[J].中国药房,2022,33(21):2622-2626.

ZHAO Fangshu,ZHANG Aijun,LIU Miaomiao,et al.Study on the effects of Jinbei oral liquid against influenza A H1N1 virus and secondary Streptococcus pneumoniae infection of mice[J].ZHONGGUO YAOFANG,2022,33(21):2622-2626.
赵方舒,张爱均,刘苗苗等.金贝口服液抗小鼠甲型H1N1流感病毒及继发性肺炎链球菌感染作用研究 ^Δ[J].中国药房,2022,33(21):2622-2626. DOI： 10.6039/j.issn.1001-0408.2022.21.11.

ZHAO Fangshu,ZHANG Aijun,LIU Miaomiao,et al.Study on the effects of Jinbei oral liquid against influenza A H1N1 virus and secondary Streptococcus pneumoniae infection of mice[J].ZHONGGUO YAOFANG,2022,33(21):2622-2626. DOI： 10.6039/j.issn.1001-0408.2022.21.11.

摘要

目的

研究金贝口服液体内抗小鼠甲型H1N1流感病毒及继发性肺炎链球菌感染的作用，为其临床应用提供参考。

方法

以磷酸奥司他韦胶囊（25.6 mg/kg）为阳性对照进行实验。通过滴鼻含0.8个半数致死量（LD

）的H1N1病毒液复制小鼠甲型H1N1流感病毒感染模型，以体质量、肺指数、肺病毒载量、肺组织病理形态变化以及血清中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6水平为指标，考察15.6、7.8、3.9 mL/kg金贝口服液体内抗甲型H1N1流感病毒的作用。通过滴鼻含0.5个LD

的H1N1病毒液和含1×10

个菌落形成单位的肺炎链球菌液复制继发性肺炎链球菌感染模型，以小鼠死亡情况、肺指数、鼻腔和肺部细菌载量以及血清中干扰素β（IFN-β）、IL-17、IL-23水平为指标，考察15.6 mL/kg金贝口服液体内抗继发性肺炎链球菌感染的作用。

结果

15.6、7.8 mL/kg金贝口服液给药6 d后，均可显著升高甲型H1N1流感病毒感染小鼠异常降低的体质量，显著降低其异常升高的肺指数及血清中TNF-α、IL-1β、IL-6水平（

＜0.05），显著降低其肺部病毒载量（

＜0.05），减轻其肺组织病变程度。15.6 mL/kg金贝口服液还可显著延长病毒-细菌共感染小鼠的存活时间（

＜0.05），降低其死亡率；显著升高其异常降低的体质量和血清中IL-17、IL-23水平（

＜0.05），显著降低其鼻腔和肺部细菌载量以及血清中异常升高的IFN-β水平（

＜0.05）。

结论

金贝口服液具有一定的抗小鼠甲型H1N1流感病毒感染作用，且能通过恢复17型免疫功能帮助机体抵抗继发性肺炎链球菌感染。

Abstract

OBJECTIVE

To study the effects of Jinbei oral liquid against influenza A H1N1 virus and secondary

Streptococcus pneumoniae

infection of rats， and to provide reference for its clinical application.

METHODS

Oseltamivir phosphate capsule （25.6 mg/kg） was used as a positive control. Influenza A H1N1 virus infection model of mice was established by nasal drops of H1N1 virus containing 0.8 median lethal dose （LD

）. The body mass， lung index， lung viral load， pathological changes of lung tissue， and serum levels of tumor necrosis factor α （TNF-α）， interleukin-1β （IL-1β） and IL-6 were used as indexes to investigate the anti-H1N1 virus effect of 15.6， 7.8 and 3.9 mL/kg Jinbei oral liquid

in vivo

. The model of secondary

S. pneumoniae

infection was established by nasal drops of H1N1 virus solution containing 0.5 LD

and

S. pneumoniae

solution containing 1×10

colony forming units. The death， lung index， nasal and lung bacterial load， serum levels of interferon-β （IFN-β）， IL-17 and IL-23 were used as indexes to investigate the effects of 15.6 mL/kg Jinbei oral liquid against secondary

S. pneumoniae

infection.

RESULTS

After 6 days of administration， both 15.6 and 7.8 mL/kg Jinbei oral liquid significantly increased the abnormally reduced body weight of influenza A H1N1 virus infected mice， significantly reduced the abnormally increased lung index and serum levels of TNF-α， IL-1β， IL-6 （

＜0.05）； it also significantly reduced the viral load in the lung （

＜0.05） and alleviated the degree of lung tissue lesions. At the same time， 15.6 mL/kg Jinbei oral liquid significantly prolonged the survival time of mice co-infected with virus and bacteria （

＜0.05） and reduced the mortality rate； it also significantly increased the abnormally reduced body weight and serum levels of IL-17 and IL-23 （

＜0.05）， while reduced the nasal and lung bacterial loads and the abnormally increased serum level of IFN-β （

＜0.05）.

CONCLUSIONS

Jinbei oral liquid has a certain anti-influenza A H1N1 virus infection effect of rats and can help the body resist secondary

S. pneumoniae

infection by restoring the type 17 antibacterial immune function.

关键词

金贝口服液甲型H1N1流感病毒肺炎链球菌继发性细菌感染

Keywords

influenza A H1N1Streptococcus pneumoniaesecondary bacterial infection

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Study on the effects of Jinbei oral liquid against influenza A H1N1 virus and secondary Streptococcus pneumoniae infection of mice

DOI：10.6039/j.issn.1001-0408.2022.21.11

摘要

Abstract

关键词

Keywords

references