Acquisition of brain mitochondria targeting compounds in <italic style="font-style: italic">Gastrodia elata</italic> and study on their neuroprotective activities

YU Xinglin; YANG Liping; CHEN Pu; DUAN Xiaohua

doi:10.6039/j.issn.1001-0408.2022.22.03

您当前的位置：

首页 >

文章列表页 >

Acquisition of brain mitochondria targeting compounds in Gastrodia elata and study on their neuroprotective activities

更新时间：2023-02-22

- Acquisition of brain mitochondria targeting compounds in Gastrodia elata and study on their neuroprotective activities
- ZHONGGUO YAOFANG Vol. 33, Issue 22, Pages: 2700-2705(2022)
- 作者机构：
  
  云南中医药大学云南省傣医药与彝医药重点实验室，昆明　650500
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.22.03
  CLC： R917;R285.5
- Published：30 November 2022，
  
  Received：17 May 2022，
  
  Revised：26 August 2022，
扫描看全文
余星霖,杨丽萍,陈普等.天麻中脑线粒体靶向化合物的获取及其神经保护活性研究 ^Δ[J].中国药房,2022,33(22):2700-2705.

YU Xinglin,YANG Liping,CHEN Pu,et al.Acquisition of brain mitochondria targeting compounds in Gastrodia elata and study on their neuroprotective activities[J].ZHONGGUO YAOFANG,2022,33(22):2700-2705.
余星霖,杨丽萍,陈普等.天麻中脑线粒体靶向化合物的获取及其神经保护活性研究 ^Δ[J].中国药房,2022,33(22):2700-2705. DOI： 10.6039/j.issn.1001-0408.2022.22.03.

YU Xinglin,YANG Liping,CHEN Pu,et al.Acquisition of brain mitochondria targeting compounds in Gastrodia elata and study on their neuroprotective activities[J].ZHONGGUO YAOFANG,2022,33(22):2700-2705. DOI： 10.6039/j.issn.1001-0408.2022.22.03.

摘要

目的

从天麻中获取脑线粒体靶向化合物，并对获取的化合物进行神经保护活性研究。

方法

提取大鼠脑组织中线粒体，通过健那绿和中性红染色评估线粒体的纯度，并通过检测线粒体膜电位（MMP）评估纯化线粒体的活性。以纯化线粒体为对照组、失活的纯化线粒体为实验组，测定并计算对照组和实验组的峰面积之差与对照组峰面积的比值（Δ

），来验证线粒体靶向化合物获取方法的可行性。通过超滤离心-高效液相色谱（HPLC）法获取天麻中脑线粒体靶向化合物并进行指认，检测其对离体线粒体活性的影响。进一步针对活性较高的化合物，采用缺氧缺糖/复氧（OGD/R）损伤HT22细胞研究其神经保护活性。

结果

提取所得的脑线粒体纯度高，具有活性。可行性验证结果显示，超滤离心-HPLC法具有识别分离功能，可用于获取具有线粒体靶向功能的化合物。从天麻中获取了2个脑线粒体靶向化合物（对羟基苯甲醇、对羟基苯甲醛），两者均可提高离体线粒体活性，其中对羟基苯甲醇活性更高。细胞实验结果显示，对羟基苯甲醇以给药浓度25、50、100 μmol/L预处理，对HT22细胞存活率影响较小。与模型组相比，对羟基苯甲醇处理可降低OGD/R损伤细胞死亡率，改善模型细胞形态，提高模型细胞ATP、MMP水平，降低模型细胞中乳酸脱氢酶、活性氧水平与线粒体通透性转换孔开放程度，大部分差异具有统计学意义（

＜0.05）。

结论

从天麻中获取2个脑线粒体靶向化合物（对羟基苯甲醇和对羟基苯甲醛），其中对羟基苯甲醇对OGD/R诱导的神经细胞损伤有明显的保护作用，可能是通过线粒体靶向治疗发挥神经保护作用。

Abstract

OBJECTIVE

To obtain mitochondria targeting compounds from

Gastrodia elata

， and to study the neuroprotective activities of these compounds.

METHODS

The mitochondria was extracted from cerebral tissue of rats. The purity of mitochondria was evaluated with Janus green staining and neutral red staining. The activity of purified mitochondria was evaluated. The purified mitochondria was included in the control group，and the inactivated mitochondria was included in the experimental group. The ratio of the difference between the peak areas of the control group and the experimental group to the peak areas of control group（Δ

） was determined and calculated to verify the feasibility of the method for obtaining the mitochondria targeting compounds. The mitochondria targeting compounds from

G. elata

were obtained by ultrafiltration centrifugation-high performance liquid chromatography （HPLC） and identified， and the effects of the compounds on the acitivities of mitochondria were also detected. The neuroprotective activities of the compounds with better activities were studied with hypoxia glucose deprivation/reoxygenation （OGD/R） damaged HT22 cells.

RESULTS

The extracted mitochondria possessed high purity and activity. Results of feasibility validation showed that ultrafiltration centrifugation-HPLC method had recognition and separation function， and could be used to obtain compounds with mitochondria targeting function. Two mitochondria targeting compounds （

-hydroxybenzyl alcohol and

-hydroxybenzaldehyde） were obtained from

G. elata

； these 2 compounds could improve the activities of mitochondria

in vitro

， and

-hydroxybenzyl alcohol had higher activity. Results of cell experiment showed that pretreatment with

-hydroxybenzyl alcohol （25， 50， 100 μmol/L） had little effect on the survival rate of HT22 cells. Compared with the model group，

-hydroxybenzyl alcohol could reduce cell mortality of OGD/R damaged cells， improve cell morphology， increase the levels of ATP and MMP， and reduced LDH and ROS levels and MPTP openness；most of the differences were statistically significant （

＜0.05）.

CONCLUSIONS

Two brain mitochondria targeting compounds （

-hydroxybenzyl alcohol and

-hydroxybenzaldehyde） are obtained from

G. elata

，among which

-hydroxybenzyl alcohol has a significant protective effect against OGD/R-induced neuronal injury， possibly through mitochondria targeting therapy.

关键词

天麻脑线粒体超滤离心高效液相色谱靶向治疗神经保护

Keywords

brain mitochondriaultrafil-tration centrifutgationHPLCtargeted therapyneuroprotec-tion

references

HE Z，NING N Y，ZHOU Q X，et al. Mitochondria as a therapeutic target for ischemic stroke[J]. Free Radic Biol Med，2020，146：45-58.

CABRAL-COSTA J V，KOWALTOWSKI A J. Neurolo-gical disorders and mitochondria[J]. Mol Aspects Med，2020，71：100826.

李振，王科，王凯强，等. 中药介导线粒体防治肝癌的研究进展[J]. 中草药，2022，53（2）：575-589.

柳威，邓林华，祁东利，等. 天麻及其有效成分的药理作用概述[J]. 中药药理与临床，2021，37（4）：240-244，12.

李云，王志伟，刘大会，等. 天麻化学成分研究进展[J]. 山东科学，2016，29（4）：24-29.

张成宸，石京山. 天麻的酚类成分及其中枢神经药理作用研究进展[J]. 中药药理与临床，2019，35（2）：167-174.

YANG X X，LIANG L，LIU X，et al. Efficiently capturing mitochondria-targeted constituents with hepatoprotective activity from medicinal herbs[J]. Oxid Med Cell Longev，2019，2019：4353791.

YANG X X，ZHOU Y Z，XU F，et al. Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method[J]. J Pharm Anal，2018，8（4）：240-249.

MENG L W，LI X K，WANG X R，et al. Amoxicillin effects on functional microbial community and spread of antibiotic resistance genes in amoxicillin manufacture wastewater treatment system[J]. J Environ Sci（China），2017，61：110-117.

DI BELLA S，LUZZATI R，PRINCIPE L，et al. Aspirin and infection：a narrative review[J]. Biomedicines，2022，10（2）：263.

CHIOZZI P，SARTI A C，SANZ J M，et al. Amyloid β-dependent mitochondrial toxicity in mouse microglia requires P2X7 receptor expression and is prevented by nimodipine[J]. Sci Rep，2019，9（1）：6475.

LEE B J，EGI Y，VAN LEYEN K，et al. Edaravone，a free radical scavenger，protects components of the neurovascular unit against oxidative stress in vitro[J]. Brain Res，2010，1307：22-27.

LUO L，LYU L，LU Y H，et al. Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells[J]. Mol Med Rep，2014，9（5）：1675-1680.

CHAIYARIT S，THONGBOONKERD V. Comparative analyses of cell disruption methods for mitochondrial isolation in high-throughput proteomics study[J]. Anal Biochem，2009，394（2）：249-258.

GEORGAKOPOULOS N D，WELLS G，CAMPANELLA M. The pharmacological regulation of cellular mitophagy[J]. Nat Chem Biol，2017，13（2）：136-146.

YU S S，ZHAO J，ZHENG W P，et al. Neuroprotective effect of 4-hydroxybenzyl alcohol against transient focal cerebral ischemia via anti-apoptosis in rats[J]. Brain Res，2010，1308：167-175.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Neuroprotective effect of sodium aescinate on rats with Parkinson’s disease by regulating SIRT1/NF-κB signaling pathway

他莫昔芬对高血压脑出血模型大鼠早期脑损伤的神经保护作用研究

FTY720对中枢神经系统影响的研究

血脂康非调脂/非心血管保护作用的研究进展

Related Author

ZHOU Huimin

CHEN Jing

OU Yidan

WANG Yulin

ZHONG Chunzheng

刘波姚鸿萍杨晓玲

岳妍殷樱虞乐华

黄金路

Related Institution

Dept. of Neurology， Danzhou People’s Hospital

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Acquisition of brain mitochondria targeting compounds in Gastrodia elata and study on their neuroprotective activities

DOI：10.6039/j.issn.1001-0408.2022.22.03

摘要

Abstract

关键词

Keywords

references