Study on the “property-effect” correlation material basis of Taohe chengqi decoction in removing blood stasis and purging heat

SHU Wanfen; LIU Wen; SONG Xinli; LIU Xingde; ZHANG Ganchun; QIN Qin; WANG Hongxin

doi:10.6039/j.issn.1001-0408.2022.22.04

您当前的位置：

首页 >

文章列表页 >

Study on the “property-effect” correlation material basis of Taohe chengqi decoction in removing blood stasis and purging heat

更新时间：2023-02-22

- Study on the “property-effect” correlation material basis of Taohe chengqi decoction in removing blood stasis and purging heat
- ZHONGGUO YAOFANG Vol. 33, Issue 22, Pages: 2706-2711(2022)
- 作者机构：
  
  1.贵州中医药大学药学院，贵阳　550025
  2.贵州医科大学药学院，贵阳　550025
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.22.04
  CLC： R965;R285.1
- Published：30 November 2022，
  
  Received：08 May 2022，
  
  Revised：21 August 2022，
扫描看全文
舒万芬,刘文,宋信莉等.桃核承气汤化瘀泻热“性-效”相关物质基础研究 ^Δ[J].中国药房,2022,33(22):2706-2711.

SHU Wanfen,LIU Wen,SONG Xinli,et al.Study on the “property-effect” correlation material basis of Taohe chengqi decoction in removing blood stasis and purging heat[J].ZHONGGUO YAOFANG,2022,33(22):2706-2711.
舒万芬,刘文,宋信莉等.桃核承气汤化瘀泻热“性-效”相关物质基础研究 ^Δ[J].中国药房,2022,33(22):2706-2711. DOI： 10.6039/j.issn.1001-0408.2022.22.04.

SHU Wanfen,LIU Wen,SONG Xinli,et al.Study on the “property-effect” correlation material basis of Taohe chengqi decoction in removing blood stasis and purging heat[J].ZHONGGUO YAOFANG,2022,33(22):2706-2711. DOI： 10.6039/j.issn.1001-0408.2022.22.04.

摘要

目的

研究经典名方桃核承气汤化瘀泻热的“性-效”相关物质基础。

方法

建立蓄血证大鼠模型，考察桃核承气汤全方（17.29 g/kg）及其药性拆分成分[苦寒组（大黄+芒硝）9.43 g/kg、辛温组（桂枝+桃仁）4.71 g/kg、甘平组（甘草）3.14 g/kg]对大鼠凝血指标（凝血酶原时间、凝血酶原活动度、活化部分凝血活酶时间、纤维蛋白原、凝血酶时间、内皮素1）和炎症指标（C反应蛋白、肿瘤坏死因子α、超氧化物歧化酶）的影响。在确定优势药性组的基础上，采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法鉴定其成分，进行分子对接并筛选结合能≤－5.0 kcal/mol的成分作为“性-效”相关物质基础。

结果

桃核承气汤全方和辛温组药材均能显著改善模型大鼠的凝血指标（

＜0.05或

＜0.01），其全方和苦寒组药材均能显著改善模型大鼠的炎症指标（

＜0.05或

＜0.01）。辛温组为化瘀优势药性组，苦寒组为泻热优势药性组。分别从辛温组、苦寒组药材中鉴定出32、35个成分；结合能≤－5.0 kcal/mol的辛温组药材成分有10个（脱落酸、羟基酪醇、原花青素B1等），苦寒组药材成分有13个（黄芩素、芦荟大黄素、去甲蟛蜞菊内酯等）。

结论

桃核承气汤全方有化瘀、泻热的功效，其物质基础分别来自辛温组药材（桂枝、桃仁）和苦寒组药材（大黄、芒硝）。

Abstract

OBJECTIVE

To study the “property-effect” correlation material basis of the classic famous prescription Taohe chengqi decoction in removing blood stasis and purging heat.

METHODS

The rat model of blood storage syndrome was established to investigate the effects of Taohe chengqi decoction （17.29 g/kg） and its property grouping components [bitter-cold property group （Rhei Radix et Rhizoma+Natrii Sulfas） 9.43 g/kg， pungent-warm property group （Cinnamomi Ramulus+Persicae Semen） 4.71 g/kg， sweet-flat property group （Glycyrrhizae Radix et Rhizoma） 3.14 g/kg] on coagulation indexes （prothrombin time， prothrombin activity， activated partial thrombin time， fibrinogen， thrombin time and endothelin-1） and inflammation indicators （C-reactive protein， tumor necrosis factor-α and superoxide dismutase） in rats. On the basis of determining the dominant property groups， ultra-performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry was used to identify the components. The molecular docking was performed， and the components with binding energy ≤－5.0 kcal/mol were selected as the basis of “property-effect” correlation material basis.

RESULTS

Taohe chengqi decoction total prescription group and pungent-warm property group could significantly improve the coagulation indexes of model rats （

＜0.05 or

＜0.01）， and the total prescription group and bitter-cold property group could significantly improve the inflammation indexes of model rats （

＜0.05 or

＜0.01）. The pungent-warm property group was the dominant property group for removing blood stasis，and the bitter-cold property group was the dominant property group for purging heat. Thirty-two and thirty-five components were identified from the pungent-warm property group and bitter-cold property group，respectively. With binding energy ≤－5.0 kcal/mol， there were 10 components （abscisic acid， 3，4-dihydroxypheny-lethanol， procyanidin B1， etc.） in the pungent-warm property group and 13 components （baicalein， aloe-emodin， demethylwedelolactone， etc.） in the bitter-cold property group.

CONCLUSIONS

Taohe chengqi decoction has the effect of removing blood stasis and purging heat， and its material basis comes from pungent-warm property group （Cinnamomi Ramulus， Persicae Semen） and bitter-cold property group （Rhei Radix et Rhizoma， Natrii Sulfas） respectively.

关键词

桃核承气汤蓄血证药性凝血指标炎症指标物质基础大鼠

Keywords

blood storage syndromemedical propertycoagulation indexinflammatory indicatormaterial basisrats

references

ZHANG J，GUO W N，LI Q，et al. Discriminant analysis of traditional Chinese medicinal properties based on holistic chemical profiling by 1H-NMR spectrometry[J]. Evid Based Complement Alternat Med，2020，2020：3141340.

陈军，姚俊宏，王裔惟，等. 基于生物药剂学思维的外用中药药性“组分-递送-效应”三要素表征思路[J]. 南京中医药大学学报，2021，37（5）：654-657.

吕春艳，吕邵娃，李国玉，等. 中药性味拆分与组合药理效应的研究进展[J]. 中国中药杂志，2018，43（14）：2892-2898.

袁培培，侯颖，李潘营，等. 基于痰饮停聚哮喘模型及主成分分析的葶苈子化学拆分组分沉降药性归属研究[J]. 中草药，2022，53（2）：449-460.

ZHOU B C，LI T，YANG M N，et al. Characterization of the hot and cold medicinal properties of traditional Chinese herbs by spontaneous photon emission ratio of mice[J]. J Ethnopharmacol，2019，243：112108.

国家药典委员会.中华人民共和国药典：一部[S].2020年版.北京：中国医药科技出版社，2020：24-290.

CHAHIN A B，OPAL J M，OPAL S M. Whatever happened to the Shwartzman phenomenon? [J]. Innate Immun，2018，24（8）：466-479.

XU J J，XU F，SHEN S J，et al. Holistic and dynamic metabolic alterations of traditional Chinese medicine syndrome in a toxic heat and blood stasis syndrome rat model[J]. RSC Adv，2017，7（89）：56471-56483.

董航，谢铱子，黄嘉华，等. 采用网络药理学研究桃仁-红花药对治疗股骨头坏死的分子机制[J]. 中国药房，2019，30（7）：917-922.

XI S Y，QIAN L C，TONG H Y，et al. Toxicity and clinical reasonable application of Taoren（Semen Persicae）based on ancient and modern literature research[J]. J Tradit Chin Med，2013，33（2）：272-279.

陈淮臣，刘文，何利，等. 桃核承气汤及其单味药对急性胰腺炎大鼠AMS、LPS、TNF-α、IL-10和NO水平的影响[J]. 时珍国医国药，2021，32（9）：2095-2098.

王伯沄. 病理学技术[M].北京：人民卫生出版社，2000：129-132.

颜永刚，王红艳，尹立敏，等. 桃仁-大黄药对对脂多糖所致蓄血证模型大鼠的影响[J]. 中药材，2016，39（5）：1148-1151.

HEBEL-GERBER S，GARCÍA-CANCINO A，URBINA A，et al. Chilean rhubarb，Gunnera tinctoria（Molina）Mirb.（Gunneraceae）：UHPLC-ESI-orbitrap-MS profiling of aqueous extract and its anti-Helicobacter pylori activity[J]. Front Pharmacol，2021，11：583961.

张开弦，姚秋阳，吴发明，等. 大黄属药用植物化学成分及药理作用研究进展[J]. 中国新药杂志，2022，31（6）：555-566.

韩迪，王姗姗，唐嵩媛，等. 基于UPLC-Q-TOF-MS技术的黄芪桂枝五物汤基准样品化学成分分析及表征[J]. 中国实验方剂学杂志，2022，28（9）：141-149.

黄琼连，周敏贤，龙江玲，等. 苦杏仁皮中膳食纤维和黄酮同步提取工艺的优化[J]. 中国药房，2022，33（6）：724-728，734.

荣晓惠，刘艳，王秋茹，等. 不同产区掌叶大黄酒蒸前后指纹图谱的建立及3种差异性成分的含量测定[J]. 中国药房，2022，33（3）：280-286.

曲中原，罗龙坦，张文君，等. 基于UPLC-Q-TOF-MS/MS和网络药理学方法探讨五味子藤茎保肝的潜在药效物质及作用机制[J]. 中草药，2022，53（8）：2407-2416.

SUN S，XUN G，ZHANG J，et al. An integrated approach for investigating pharmacodynamic material basis of Lingguizhugan Decoction in the treatment of heart failure[J]. J Ethnopharmacol，2022，295：115366.

XIA W W，HU S S，WANG M M，et al. Exploration of the potential mechanism of the Tao Hong Si Wu Decoction for the treatment of postpartum blood stasis based on network pharmacology and in vivo experimental verification[J]. J Ethnopharmacol，2021，268：113641.

谢军，彭宝虹. 探究黄芪桂枝五物汤治疗糖尿病周围神经病变经验[J]. 中医临床研究，2021，13（1）：84-86.

李玉洁，张为佳，Mayuree Tantisira，等. “苦寒”方药性效解[J]. 中医杂志，2014，55（19）：1630-1634.

郝二伟，邓家刚，杜正彩，等. 平性活血化瘀药对寒、热2种血瘀证双向适用的实验研究Ⅱ[J]. 中国中药杂志，2013，38（4）：605-610.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on metabolites derived from Zhideke granules in rats in vivo

Effects of Yiru tiaojing granules on the pharmacokinetics of olanzapine in rats

Improvement effects of Shangke xiaoyan hydrogel plaster on osteoarthritis model rats

Study on protective effects of twin drugs of tetramethylpyrazine-scutellarein on cerebral ischemia-reperfusion injury model rats and its mechanism

Intervention effect and mechanism of Zhongfeng yure decoction on ischemic stroke model rats

Related Author

LIANG Jie

ZHENG Piaoxue

CHEN Huihua

HUANG Chunyan

LIANG Yanli

LU Chunlian

XIE Jingjing

MA Yuming

Related Institution

Dept. of Pharmacy， Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine

Qinzhou Inspection and Testing Center

Guangxi Zhuang Yao Medicine Engineering Technology Research Center

National Engineering Research Center South China Branch of TCM Solid Preparation Manufacturing Technology

Guangxi Universities Laboratory of TCM Extraction， Purification and Quality Analysis

Address：8/F,129 Daping Main Street,Yuzhong District,Chongqing 400042,P.R.China
Technical support is provided by Beijing Founder Electronics co., LTD 渝ICP备15012710号公网安备50010302001817
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰